Lab Services

 

Lab Services

Hemogram lab

1. Haemogram including Haemoglobin, TLC,DLC, Platelet count, MCV, MCH, RDW, Haematocrit, peripheral smear, reticulocyte count.

2. Bone marrow morphology from bone marrow aspirate and bone marrow touch smear (Jenner-Giemsa stain)

3. Bone marrow biopsy (haematoxylin eosin stain)

 Coagulation Lab

1. Screening coagulogram

   a. Prothrombin time

   b. Activated partial thromboplastin time

   c. Thrombin Time

   d. Fibrinogen

   e. Clot solubility test

   f. D-Dimer

2. Factor assay for – Factor VIII, IX, X, II, V, XI

3. Inhibitor screening

4. Bethesda assay for Factor VIII,IX inhibitor titre

5. Platelet function test by Light transmission aggregometry

6. INR for monitoring of oral anticoagulant therapy

7. Lupus anticoagulant workup

8. Thrombophilia work up [ Antithrombin, Protein C , Protein S, Activated protein C Resistance]

9. VWD antigen assay

10. Beta 2 glycoprotein antibodies (IgG and IgM) testing

Anemia Lab

1. High performance liquid chromatography (HPLC) for detection of abnormal haemoglobins & their quantitation including HbF and A2

2. Sickling test

3. Serum iron studies- S. iron , TIBC, % saturation

4. Coomb’s test - direct by gel technology

5. Ham’s test and sucrose lysis test for PNH

6. Heinz bodies /HbH inclusion

7. G6PD screening test

8. Osmotic fragility test

9. Plasma haemoglobin

10. Urine haemosiderin

11. Cryoglobulin testing

Flow cytometry Lab

1. Immunophenotyping for acute leukemia and chronic lymphoproliferative disorders

2. Measurable residual disease in ALL, AML

3. Immunophenotyping for plasma cell neoplasm and MRD in MM

4. Flow cytometry for monocyte subset analysis

5. PNH study on PB by FLAER

6. EMA dye binding assay for hereditary spherocytosis

7. CD34 stem cell enumeration

8. Double negative T cells for ALPS

9. Platelet flow cytometry for GT and BSS

10. T cell subsets for L-HES

Molecular lab

1. Thrombophilia mutations

   a. Factor V leiden mutation

   b. Prothrombin P20210 G®A mutation

   c. MTHFR C6 77T gene polymorphism

2. Haemophilia

   a. Intron 22 inversion

   b. Intron 1 inversion

3. Carrier detection and prenatal diagnosis using CVS sample by linkage analysis

4. Thalassemia

   a. Alpha thalassemia mutation- 3.7 and 4.2 deletions

   b. Alpha thalassemia triplication

   c. GAP-PCR for Delta-beta Thalassemia (Asian Indian inversion deletion)

   d. ARMS PCR for common beta thalassemia mutations

5. RTPCR for BCR-ABL1 (qualitative)

6. RQPCR for BCR-ABL1 (quantitative)

7. JAK2V617F mutation by ARMS PCR

8. CALR (Exon-9)

9. PMR-RARa (Qualitative) by RTPCR

10. FLT3- ITD by RTPCR (qualitative)

11. Inv 16 by RTPCR for (qualitative)

12. RUNX1::RUNX1T1 fusion by RTPCR for (qualitative)

Immunohistochemistry

1. Acute leukemia- subtyping and follow up

2. Lymphoma- Hodgkin, Non-Hodgkin

3. Evaluation of mast cell disorders

4. Anemia evaluation- PRCA, MDS

5. Multiple Myeloma workup

Cytochemistry

Sudan black (SB)

Myeloperoxidase (MPO)

Leukemia alkaline phosphatase (LAP)

PAS

NSE (Non-specific esterase)

Acid phosphatase

Double esterase