Hospital Statistics 2015-16

Dr. MANISH JAIN

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अंतिम बार अपडेट हुआ बुधवार, 14 अक्टूबर 2020 06:40

             2099/2083- Department of Reproductive Biology

          AIIMS, New Delhi – 110029

1. Research Area & Interest:    Reproductive Genetics including developmental genetics & preimplantation genetics, Genomic and 

                                                     Epigenomic of Male  & Female Infertility, Semen Banking & Molecular   Cytogenetics

2. Education Details (Post-graduation onwards & Professional Career)  

3.  Employment Details: Position and Employment (Starting with the most recent  employment)  

          4. Details of ongoing projects.   As PI:

• High resolution Genomic Screening using SNP Microarray to find out Genomic Causes  in Idiopathic Sertoli Cell Only Syndrome (SCOS) cases (ongoing: funding by AIIMS, New Delhi, Grant: 10 lakhs).
• Idiopathic Sertoli Cell Only Syndrome (SCOS): An Investigation to find out Epigenomic Etiologies (ongoing: funding by ICMR, New Delhi, Grant: 50 lakhs).

     As Co-PI

• An investigation to find out genetic factors in idiopathic familial ovarian failure. (ongoing: funding by DST, New Delhi, Grant: 45 lakhs).
• Poly Cystic Ovarian Syndrome (PCOS): An investigation to find out reasons for discrepancy between hyperandrogenism (clinical) & hyperandrogenemia (biochemical) and underlying etiologic (epigenetic & genetic) factors (ongoing: funding by DST, New Delhi, Grant: 45 lakhs).

5. Departmental projects (including thesis/dissertations)  

      Thesis Submitted

        Ongoing

• Worked on Reproductive Endocrinology/Reproductive Genetics/Male Infertility, Microarray Technologies.
• Worked on “Rapid detection of Chromosome 21 & 13   Aneuploidy by Primed In Situ Labelling”.
• Worked on “Genetic and Endocrine study on Primary Testicular Failure”.         
• Worked on “Sertoli Cell Maturation Status, Role of Heavy Metals & Role of Vitamin A in Spermiation Defects in Human”.                                                                                                                                                                              
• Worked on “Prevalence of 22q11.2 micro deletion syndrome in children with structural cardiac malformation: a tertiary care referral hospital-based study in Delhi”.
• Got training in Array CGH, MLPA, QF-PCR techniques in a workshop conducted by Department of Genetics, AIIMS in February, 2011.
• Got training in Array CGH in “The Centre for Genomic Application” (TCGA) in 2010.
• Training on Genetic analyzer on 18^th- 20^th March, 2013at Gurgaon facility of Applied Biosystem 310.
• Mouse embryo production, handling and FISH
• Co-Organizer in symposium on “Impact of Endocrine Disrupters on Reproductive Health” conduct on 20^th July,2013 in Department of Reproductive Biology, AIIMS
• Organize a “National Workshop on Molecular Cytogenetics: In House FISH Probe Production”12-16th November, 2018, Department of Reproductive Biology, AIIMS New Delhi 
• 7. Professional Recognition/Awards/Prize/Fellowship:

8. Service Offering:  

    Microdeletion FISH on blood (Interphase/ Metaphase cell), buccal cell, urine cell,  solid tissue etc.

• Digeorge syndrome (22q11.2)
• William syndrome (7q11.23)
• Prader willi syndrome (15q11.13)
• Retenoblastoma  (13q)
• Miller decker syndrome (17p13.3)
• Langer Giedions syndrome  (8q24.11)
• 1p36.13
• Wolff  Hirschhorn (4p16.3)

Prenatal FISH (Amniotic fluid cells/chorionic tissue)  

• Trisomy 21 (Down syndrome)
• Trisomy 18 (Edward Syndrome)
• Triomy 13 (Patau Syndrome)
• Any autosomal aneuploidies (on request)

Karyotyping (only research use)  

Sex Chromosome FISH (X, Y, SRY: only research & infertility)  

Yq Microdeletion PCR (Cover app. 90%)  

PRINS (Chromosome13, 18, 21, X, Y)  

QF-PCR (DG, WS, PWS, RB, MD, Langer-Giedions, Trisomy 13, 18, 21, etc.)   

DNA Microarray

• WGA of Microdeletion Syndrome cases through Microarray

• Primary Testicular Failure / POF/PCOS cases

Short term Training on Molecular Cytogenetics Techniques

• Sharma A, Jain M, Halder A, Kaushal S. Intra-individual genomic variation study in tissues (Blood vs. Testis) through SNPs Microarray: Case report in two idiopathic Sertoli cell only Syndrome patients (SCOS). Reproduction & Infertility (Accepted Feb. 2020, Manuscript ID: JRI-1909-1647 (R1).
• Manisha Singh, Manish Jain, Samrat Bose, Ashutosh Halder, Tapas Chandra Nag, Amit Kumar Dinda, Sujata Mahanti. 22(R)-hydroxycholesterol for differentiation of MSCs & amelioration of Parkinsonian symptoms in rats. The EMBO Journal. 2020 (submitted)
• Jain M, Halder A, et al. Identification of Genomic imbalances (CNVs as well as LOH) in Sertoli Cell Only Syndrome cases through Cytoscan Microarray. Andrology 2020(submitted)Halder A, Chaudhary I, Jain M. Sex Ratio Trajectory in Mouse. Syst Biol Rep Med 2020 (submitted)
• Jain P, Wadhwa N, Joshi MK, Jain M, Halder A, Mishra K. Cellular mesenchymal epithelial transition (C‑MET) gene copy number variation in gastric adenocarcinoma: A pilot search for new marker for targeted therapy in HER‑2/neu resistance. Indian J Pathol Microbiol 2020; 63:86-9.
• Kalsi AK, Halder A, Jain M, Chaturvedi PK, Mathew M, Sharma JB. Association of raised levels of IL-4 and anti-TPO with hyperprolactinemia. Am J Reprod Immunol. 2019 Jan 4:e13085. doi: 10.1111/aji.13085Kalsi AK, Halder A, Jain M. Association of Cadmium, Chromium, Manganese and Lead with hyperprolactinemia. JCDR(Accepted Dec. 2019, Ref no: 42690)
• Kalsi AK, Halder A, Jain M, Chaturvedi PK, Sharma JB. Prevalence and Reproductive Manifestations of Macroprolactinemia. Endocrine. 2018Sep 29. doi: 10.1007/s12020-018-1770-6Kumar P, Jain M, Kalsi AK, Halder A. Molecular characterization of a case of dicentric Y presented as nonobstructive azoospermia with testicular early maturation arrest. Andrologia. 2018 Mar; 50(2). doi: 10.1111/and.12886
• Halder A, Kumar P, Jain M, Iyer VK. Copy number variations in testicular maturation arrest. Andrology 2017; Feb 19:1-13.
• Halder A, Kumar P, Jain M, Kalsi AK Genomics: Tool to predict and prevent male infertility. Frontiers In Bioscience, Scholar 2017; 9:448-508.
• Halder A, Kumar P, Jain M. Primary testicular failure: A search for ideal biomarkers. Indian J Endocr Metab. 2017; 21.
• Halder A, Jain M, Kalsi AK. SNP Microarray in FISH Negative Clinically Suspected 22q11.2 Microdeletion Syndrome. Scientifica (Cairo). 2016; 2016:5826431.
• Jain M, Kalsi AK, Srivastava A, Gupta YK, Halder A. High Serum Estradiol and Heavy Metals Responsible for Human Spermiation Defect-A Pilot Study.J Clin Diagn Res. 2016Dec; 10(12):RC09-RC13.
• Halder A, Jain M and Kumar P. Primary Testicular Failure: An Overview J Clin Diagn Res 2015; 3:1
• Halder A, Jain M and Chaudhary I. Suspected microdeletion syndromes and molecular cytogenetic techniques: an experience with 330 cases. Molecular Cytogenetics 2014; 7(Suppl 1):O7.
• Chaudhary I, Jain M, Halder A. Sperm Sex Ratio (X: Y Ratio) and its Variations. Austin J Reprod Med Infertil 2014; 1:1.
• Jain M, Mohan V, Chaudhary I, Halder A. Sertoli cell only syndrome and glaucoma in a SRY positive XX infertile male. JCDR; 2013:7 (7): 1457-1459A. Halder and Jain M Cytogenetics to Cytogenomics: Transition from Chromosome to DNA Sequence. Global Journal of Human Genetics & Gene Therapy.2013; 1: 90-104
• Halder A, Jain M, Chaudhary I, Kumar G, T. Das & Y. K. Gupta “Dark-coloured semen in nonobstructive azoospermia: a report of four cases.” Andrologia 2013; Feb 28. doi: 10.1111 / and. 12078.
• Halder A, Jain M, Chaudhary I "Rapid Detection of Chromosome X, Y, 13, 18 & 21 Aneuploidies by Primed In Situ Labeling/Synthesis (PRINS) Technique." Indian Journal of Human Genetics 2013; 19 (1): 14-17.Halder A, Jain M, Chaudhary I, Gupta N and Kabra M. Fluorescence in-situ hybridization (FISH) using non-commercial probes in the diagnosis of clinically suspected microdeletion syndromes: an experience with 301 cases. IJMR 2013; 138: 135-142.
• Halder A, Jain M, Chaudhary I, Varma B Chromosome 22q11.2 microdeletion in monozygotic twins with discordant phenotype and deletion size.  Molecular Cytogenetics 2012;5:13.Halder A, Jain M, Chaudhary I, Kabra M. Prevalence of 22q11.2 microdeletion in 146 patients with cardiac malformation in a referral hospital of North India. BMC Medical Genetics 2010; 11: 101.
• Yadav N, Kanjirakkuzhiyil S, Kumar S, Jain M, Halder A, Saxena R, Mukhopadhyay A. The therapeutic effect of bone marrow-derived liver cells in the phenotypic correction of murine haemophilia A.Blood 2009 Nov 12; 114(20): 4552-61.
• Halder A, Jain M, Kabra  M, Gupta N. Mosaic 22q11.2 micro deletion syndrome: diagnosis and clinical manifestations of two cases. Halder Molecular Cytogenetics 2008; 1:18 doi: 10.1186/1755-8166-1-18.
• Rishi I, Halder A, Sharma JB, Jain M, Sharma M. Single Strand Conformation Polymorphism and Sequencing of HS6ST2 Gene in Patients of Idiopathic Premature Ovarian Failure. Journal of Clinical and Diagnostic Research. 2020; 14(2): GC01-GC08.
• Jain P, Wadhwa N, Joshi MK, Jain M, Halder A, Mishra K. Cellular mesenchymal epithelial transition (C-MET) gene copy number variation in gastric adenocarcinoma: A pilot search for new marker for targeted therapy in HER-2/neu resistance. Indian J Pathol Microbiol. 2020 Jan-Mar; 63(1): 86-89. doi: 10.4103/IJPM.IJPM_396_19. PubMed PMID: 32031129.
• Halder A, Kumar H, Kalsi AK, Jain M. Dihydrotestosterone: a potential biomarker of hyperandrogenaemia in PCOS. 2020 Reprod Med and Biol (submitted)
• Halder A, Jain M, Kalsi APK. Mosaicism in 22q11.2 Microdeletion Syndrome. J Clin Diagn Res. 2018; 12 (11): GC01-GC06  
• Halder A & Jain M. Molecular Cytogenetics Manual. 2012.  

10. Book Chapters 

1. Jain M, Kalsi AK, Kumar P, Halder A. The Human Y chromosome. Basics of Human Andrology-A Textbook, Springer. 2017 Jul: 77-98. DOI: 10.1007/978-981-10-3695-8_7.
2. Jain M, Kumar P, Venkateswaran Iyer, Halder A. Primary Testicular Failure. Basics of Human Andrology-A Textbook, Springer. 2017 Jul: 417 436. DOI: 10.1007/978-981-10-3695-8_24.

11. Published Abstracts  

1. Kalsi AK, Halder A, Jain M. An investigation on bone mineral density in hyperprolactinemia. Indian Journal of Endocrinology and Metabolism 2019; 23 (suppl 1): pp. S36 (IJEM_314_19)
2. Sharma A, Jain M, Halder A, Kumar Rajeev, Kaushal SeemaSNP Microarray study in Sertoli Cell Only Syndrome (SCOS). ISSRF, JNU, New Delhi, 22-25 Feb 2019.
3. Kalsi AK, Halder A, Jain M, Srivastava A, Sharma JB. Association of Cadmium, Chromium, Manganese and Lead with  hyperprolactinemia. ISSRF, JNU, New Delhi, 22-25 Feb 2019.
4. Halder A, Kumar H, Kalsi AK, Jain M. Dihydrotestosterone (DHT): a potential biomarker of hyperandrogenaemia in polycystic ovary syndrome. Ind J End Metab 2018 suppl. IJEM_429_18R2.
5. Kalsi AK, Jain Manish, Sharma JB, Chaturvedi PK, Srivastava A, Halder A. Macroprolactinemia: Prevalence and etiopathologic associations. Gynecological endocrinology, the 18th World Congress; Florence, Italy, 7-10March 2018.
6. Halder A, Kumar P and Jain M. Primary testicular failure: a search for ideal biomarkers. Ind J End & Meta 2017; 21 (suppl 1): pp. S2. March issue (IJEM_420_16)
7. Halder A, Kumer H, Kalsi AK, Jain M. Polycystic ovary syndrome: The pros and cons of various diagnostic criteria and investigation to find out associations of various factors implicated with PCOS. Ind J Endoc Metab 2017 suppl (poster no. 123; IJEM_466_17)
8. Kalsi AK, Jain M, Halder A. Macroprolactinemia: prevalence and etiopathologic associations. Ind J End Metab 2017 suppl (poster no. 144; IJEM_482_17)
9. Halder A, Kumar P and Jain M. Genomic and epigenomic factors in male infertility. In: Cross breed male infertility and bovine genomics. Edited by De S, NDRI, Karnal, India. 2016;pp.80.(http://cbp.icar.gov.in/Data/Coordinator/7100/Mannual%20S%20De%20Cross % 20 breed%20male%20infertility.pdf)
10. Halder A, Jain M, Chaudhary I, Mohan V, Kumar P. Suspected microdeletion syndromes and molecular cytogenetic techniques: an experience with 330 cases. Molecular Cytogenetics 2014; 7 (Suppl 1): 20 (O7). DOI: 10.1186/1755-8166-7-S1-O7. ISSN: 1755-8166.
11. Halder A, Jain M, Kumar P. Primary Testicular Failure: Genotype Phenotype Correlation of 140 cases. Andrology 2014; 2 (suppl. 1): 66-67 (poster no.16).
12. Jain M, Halder A. A Preliminary Study on Human Spermiation Defect. Ind J Hum Genet 2014; 20 (suppl 1): S116.
13. Halder A, Jain M, Chaudhary I, Mohan V, Kumar P. Suspected microdeletion syndromes and molecular cytogenetic techniques: an experience with 330 cases. In 63^rd annual conference of ASHG 2013, Boston, USA (3246T), p583
14. Jain M, Halder A. Sertoli cell only syndrome: Status of sertoli cell maturation and function. Indian J Endocr Metab 2012; 16: S514-515.

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