Confocal Imaging Facility
The Confocal Imaging Facility in the Department of Physiology is equipped with a Leica TCS SP2 AOBS with an inverted microscope DMIRE2 having 6 visible laser lines (458, 476, 496, 514, 546, 633 nm) and 2 UV laser lines (351, 364 nm). It has 4 PMTs to collect emitted light and 1 PMT to collect transmitted light.
Isolated human endometrial epithelial and stromal cells grown on collagen 3-D biomatrix showing evidence of gland formation in vitro. A: Transmitted image; B: Epithelial cell nuclei stained with DAPI; C: Transmitted overlaid image of immunostaining for vascular endothelial growth factor (VEGF); D: Glandular cell immunostaining for VEGF using anti-VEGF Pab & Alexa fluor 633.Leica SP2 AOBS has z-sectioning ability for 200 mm
Serial Z sectioning of human endometrial gland grown in vitro showing localization of intracellular VEGF immunostaining
Colocalization of biomolecules using Leica SP2 AOBS
Isolated human endometrial epithelial and stromal cells grown on 3-D collagen biomatrix expressing progesterone receptor (B) and insulin like growth factor binding protein-2 (C). Cell nuclei visible from DAPI staining (A), PR and IGFBP-2 colocalized in endometrial cells (D). Immunostaining for PR using anti-PR Mab & Alexa Fluor- 488; for IGFBP2 using anti-IGFBP-2 Pab & Alexa Fluor- 633.
Cytoplasmic distribution of viral proteins tagged with enhanced green fluorescent protein
Cytoplasmic expression of Hepatitis E virus fusion protein (A), pORF1EGFP and pORF2EGFP (B) in transfected HepG2 cells. FRET analysis programme is available for further studies.
Contact Details:- Prof. D.Ghosh, Department of Physiology, AIIMS, New Delhi-29. Room No. 2005. Tel No. 26594625.