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अखिल भारतीय आयुर्विज्ञान संस्थान, नई दिल्ली
All India Institute Of Medical Sciences, New Delhi
कॉल सेंटर:  011-26589142

Dr. Pramod Kumar Gautam

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Name of Faculty Member: Dr. Pramod Kumar Gautam

Designation: Assistant Professor

Address: Room No 3027-B, Teaching block, Department of Biochemistry, 
                All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110029

Official Phone: 011-26546514

Email id: यह ईमेल पता spambots से संरक्षित किया जा रहा है. आप जावास्क्रिप्ट यह देखने के सक्षम होना चाहिए. ; यह ईमेल पता spambots से संरक्षित किया जा रहा है. आप जावास्क्रिप्ट यह देखने के सक्षम होना चाहिए.

Skype id -Pramod_kumar_gautam

https://www.researchgate.net/profile/Pramod_Gautam3

Short Biography

Academic Details

Degree

Subject

University/ Institution

Year

B.Sc.

Chemistry, Botany, Zoology

D. D. U. Gorakhpur University, Gorakhpur

2004

M.Sc.

Biochemistry

V.B.S.PU. Jaunpur

2006

Ph.D.

Biochemistry

Banaras Hindu University

2014

 

 

Positions held:

Position

Department

University/ Institution

Dates

Assistant Professor

Biochemistry

AIIMS, New Delhi

December2016-present

Post Doc Fellow

Biochemistry

Banaras Hindu University, Varanasi

2014-2016

 

GRANTS AND FELLOWSHIPS:

  • 2016-DST Research Grant (ongoing)

Award/Honors

  • Best Poster Presentation Awarded, International Symposium on Cancer Biology, Nov 14-16, 2011, National Institute of Immunology, New Delhi, India.
  •  DST Inspire Faculty award in the year 2016

 

Membership of Society

  • Life Member of The Science Advisory Board
  • Life member of the Indian Immunology Society
  • Member of the International Society of Exercise and Immunology
  •  Member of the American Academy of Allergy, Asthma & Immunology (AAAI).
  • Member of the Clinical Immunological Society.

o   Life Member of Cancer Immunotherapy (CIMT), Germany

    Editorial Board Membership

  • RED FLOWER PUBLICATION Ltd In the 2016
  • Member of Editorial Board of IRA publication form 2016

 

Research Focus of the Lab

  • Modulating and targeting HSP70 in lymphoma
  • Implication of Nanoparticles-Peptide Complex in Cancer Therapy
    • Targeting Stem cell for immunotherapy
    • Developing HSP based diagnostic Kit for Pre-stage of diagnosis of diseases.
    • Targeting cell  based ( Mɸ/DCs/NK cell/Treg)  immunotherapy

 

Positions Available

We are seeking applications from motivated, goal-oriented individuals with excellent academic background and having their own fellowships. Candidates with experience in Immunology/ Cancer Biology/Immunotherapy/ Drug delivery are encouraged to contact and send their CV along with a cover letter at  यह ईमेल पता spambots से संरक्षित किया जा रहा है. आप जावास्क्रिप्ट यह देखने के सक्षम होना चाहिए.

 Key publications

  1. Gautam PK, Deepak P, Kumar S, Acharya A. Role of macrophage in tumor microenvironment: prospect in cancer immunotherapy. Euro. J. Inflammation. (2013) 10(1): 1-14.
  2. Gautam PK, Maurya BN, Kumar S, Deepak P, Kumar S Jr, Tomar MS, Acharya A Progressive growth of a murine T cell lymphoma alters population kinetics and cell viability of macrophages in a tumor-bearing host. Tumor Biol.  (2013). 34:827–836.
  3. Kumar S, Deepak P, Kumar S, Gautam PK, Acharya A.  A benzophenanthridine alkaloid, chelerythrine induces apoptosis in vitro in a Dalton’s Lymphoma.. J Cancer Res Ther. (2013) 9(4):693-700.
  4. P. K. Gautam & Arbind Acharya. Morphological effects of autologous hsp70 on peritoneal macrophages in a murine T cell lymphoma. Tumor Biol., (2013). 34:3407–3415.
  5. Pramod K. Gautam & Arbind Acharya Suppressed expression of homotypic multinucleation, extracellular domains of CD172α (SIRP-α) and CD47 (IAP) receptor in TAMs up-regulated by Hsp70-peptide complex in Dalton’s lymphoma.. Scan J Immunol.,(2014). 80; 22-35.
  6. Pramod Kumar Gautam & Arbind Acharya. Antigenic Hsp70–peptide upregulate altered cell surface MHC class I expression in TAMs and increases anti-tumor function in Dalton’s lymphoma bearing mice.  Tumor Biol., (2015). 36:2023–2032.
  7. Pramod K. Gautam & Arbind Acharya Antigenic Hsp70-peptide upregulate altered suppressed expression of docking receptor ICAM-1 in TAMs increases in Dalton's lymphoma bearing mice.. IJRST., (2015)., 5(III) :86-105.
  8. Pramod K. Gautam & Arbind Acharya Suppressed expression of CD80 (b7.1) and CD86 (b7.2) receptors in tams up-regulated by autologous hsp70– peptide complex in dalton’s lymphoma bearing balb/c mice IJRST., (2015)., 5(III):106-127.
  9. Pramod Kumar Gautam, Sanjay Kumar, Arbind Acharya. Non-mammalian Animals as Experimental Models for Modern Scientific Research. IJAREAS. (2015). 67-76.
  10. Sanjay Kumar, Pramod Kumar Gautam, Munendra Singh Tomar, Arbind Acharya. CD28 mediated T cell responses is upregulated by exogenous application of autologous hsp70-peptide complex intumor bearing mice. Immunol Res (2016) 64:313–323.

Ongoing projects:

  1. Role of Macrophage in the regulation of Bone and Blood forming stem cell and progenitor cells migration and development” (DST Funded)2017-07-31

Dr.Riyaz Ahmad Mir

Name of Faculty Member: Dr.Riyaz Ahmad Mir

Designation: Assistant Professor

Address: 3rd floor Dept. of Biochemistry(Room no 3027-B)

Official Phone:01126546514

Email id: यह ईमेल पता spambots से संरक्षित किया जा रहा है. आप जावास्क्रिप्ट यह देखने के सक्षम होना चाहिए.

Academic Details

Degree

Subject

University/ Institution

Year

B.Sc.

Zoology, Botany, Chemistry, English

University of Kashmir

Hazratbal Srinagar

2002

M.Sc.

Biochemistry

University of Kashmir

Hazratbal Srinagar

2004

Ph.D

Biochemistry

AIIMS New Delhi

2011

 

Positions held:

 

Position

Department

University/ Institution

Dates

Postdoc Research Associate

Genetics Cell Biology and Anatomy

University Of Nebraska Medical Center Omaha NE,USA

2011-2014

Senior Research Associate

Genetics Cell Biology and Anatomy

University Of Nebraska Medical Center Omaha NE,USA

2014-2016

Assistant Professor

Biochemistry

AIIMS New Delhi

Nov2016—present

 

Awards and Society Memberships

Carl Storm international Diversity Fellowship (2009).

Research Focus of the Lab

The research focus of my lab is to understand how molecular chaperones regulate cell cycle , and how this machinery is disrupted during cancer. Another area of my research is how oncoproteins disrupt chaperone function.

Positions Available

Candidates with their own fellowship are welcome to join my lab

Key publications

  1. MohdSaleem Dar, Paramjeet Singh, Riyaz A. Mir,Mohd Jamal Dar.(2017) “Βeta­cateninN­terminaldomain:An enigmatic region prone to cancer causing mutations” Mutation Research Reviews in Mutation Research 773 : 122-33.
  2. Olou AA, Sarkar A, Bele A, Gurumurthy CB, Mir RA, Ammons SA, Mirza S, Saleem I, Urano F, Band H, Band V.(2017) “Mammalian ECD protein is a novel negative regulator of the PERK arm of unfolded protein response.”Mol Cell Biol Epubahead of print.
  3. Riyaz A. Mir, Jeff Lovelace, Nicholas P. Schafer, Peter D. Simone, AdmirKellezi, Carol Kolar, GaelleSpagnol,Paul L. Sorgen, Hamid Band, Vimla Bandand Gloria  E.O.Borgstahl. (2016) “Biophysical characterization and modeling of human Ecdysoneless (ECD) protein supports a scaffolding function.” AIMS BIOPHYSICS 3 (1): 146-162
  4. Mir RA, Bele A, Mirza S, Srivastava S, Olou A, Ammons SA, Kim JH, Gurumurthy CB, Qiu F, Band H, Band V. (2015) “A novel interaction of ECD protein with R2TP complexcomponent RUVBL1 is required for the functional role of ECD in cell cycle progression”.Mol Cell Biol.36(6):886-99.
  5. 5.      Belle A, Mirza S, Zhang Y, Ahmad Mir R, Lin S, Kim JH, Gurumurthy CB, West W, Qiu F, Band H, Band V. (2015)“The cell cycle regulator ecdysoneless cooperates with H-Ras to promote oncogenic transformation of human mammary epithelial cells”.Cell Cycle.14(7)990-1000
  6. 6.      Chaturvedi NK, Mir RA, Band V, Joshi SS, Guda C (2014)“Experimental validation of predicted subcellular localizations of human proteins”. BMC Res Notes 7:992-997
  7. Romsha Kumar, VishwasTripathi, Syed M Andrabi, NeeraNath, Riyaz A Mir, Shyam S Chauhan, KalpanaLuthra.CXCR7 mediated Giα independent activation of ERK and Akt promotes cell survival and chemotaxis in T cells. (Cell Immunol.  2012; 272(2): 230-41)
  8. Riyaz A Mir, Shyam.S Chauhan.Down regulation of a matrix degrading cysteine protease cathepsin L, by acetaldehyde: role of C/EBPα (PloS One2011; 6(6): e20768).
  9. Shivani Mittal, Riyaz A Mir, and Shyam S. Chauhan. Upregulation of human cathepsin L expression by transcriptional regulatory mechanism. (Biol. Chem.2011; 392(5):405-13).
  10. Riyaz A Mir*, R Katara *, A AShukla, N Singh,Shyam.SChauhan.Wild   Type p53-Dependent Transcriptional up Regulation of Cathepsin L Expression is mediated by C/EBPα in Human Glioblastoma Cells. (Biol Chem. 2010; 391(9):1031-40) * Equal authorship.

 

Dr. Pragyan Acharya

Left to right: K. David Raja, Dr. Pragyan Acharya, AkshayMunjal

 

Address: Room No. 3002,

                Teaching Block,

               AIIMS, New Delhi-29.

Phone:011- 26546431

Email id:dr.pragyan.acharyaATgmail.com

Social media links: https://in.linkedin.com/in/pragyan-acharya-40599b16

Academic Details:

Degree

Subject

University/ Institution

Year

Ph.D.

Biochemistry

Indian Institute of Science, Bangalore

2011

M.S.

Biological Sciences (specialization Biochemistry)

Indian Institute of Science, Bangalore

2004

B.Sc. (H)

Biochemistry

Sri Venkateswara College, Delhi University

2001

 

 

Positions held:

 

Position

Department

University/ Institution

Dates

Assistant Professor

Biochemistry

AIIMS, New Delhi

October 2015-present

Visiting fellow

NIAID

National Institutes of Health, USA

February 2012-October 2015

 

 

GRANTS AND FELLOWSHIPS:

  • 2016-DST Early Career Award
  • 2016 AIIMS- Intra Mural Grant
  • Grant for 2017 EMBO Global Exchange Lecture Course entitled Malaria Genomics and Public Health.

AWARDS AND HONORS

  • Fellows Award for Research Excellence 2015- sponsored by the NIH Scientific Directors, the Office of Intramural Training & Education and FelCom/NIH.
  • Fellowship in the Khorana Nirenberg Scholars Program (US-India Career Transition Award- sponsored by DBT, India and NIH, USA) 2011
  • Ranbaxy Science Scholar 2010
  • Integrated Ph.D. Fellowship by Indian Institute of Science: 2001-2009 

SOCIETY MEMBERSHIP

Indian Society of Translational Research

 

Research Focus of the Lab

The research focus of our laboratory is the identification of metabolite, protein and nucleic acid based biomarkers that have diagnostic or prognostic value in different diseases. At the moment, we are studying the following conditions specifically-

(1) Celiac disease- biomarker identification through proteomics approaches.

(2) Organ Failure in Acute-on-chronic liver failure: biomarker identification through metabolomics approaches.

(3) Severe P. falciparum and P. vivax malaria: A systems biology approach towards understanding pathogenesis and identifying metabolite biomarkers of severe disease.

When it is appropriate, the mechanisms of disease pathogenesis will also be explored.

 

List of Lab members (Including technicians and attendants)

1. K. David Raja, M.Sc. Student

2.AkshayMunjal, M.Sc. Student

3.Poornima Gola, Technician

 

Positions available currently:

  • 1 motivated JRF is required to work on the project titled “Investigating the association of Purine Salvage Pathway with Severe Malaria Outcomes”.
  • I am happy to consider short term trainees.

 

Key publications

 

Research Papers 

1. Acharya P, Chaubey S, Grover M, Tatu U. An Exported Heat Shock Protein 40 Associates with Pathogenesis-Related Knobs in Plasmodium falciparum Infected Erythrocytes. PLoS One. 2012;7(9):e44605. Epub 2012 Sep 7.

2. Acharya P*, Pallavi R*, Chandran S, Dandavate V, Sayeed SK, Rochani A, Acharya J, Middha S, Kochar S, Kochar D, Ghosh SK, Tatu U. Clinical proteomics of the neglected human malarial parasite Plasmodium vivax. PLoS One. 2011;6(10):e26623.  Epub 2011 Oct 20. PubMed PMID: 22028927; PubMed Central PMCID: PMC3197670.*These authors have contributed equally.

3. Acharya P*, Pallavi R*, Chandran S, Chakravarti H, Middha S, Acharya J, KocharS,  Kochar D, Subudhi A, Boopathi AP, Garg S, Das A, Tatu U. A glimpse into the clinical proteome of human malaria parasites Plasmodium falciparum    and    Plasmodium    vivax.    Proteomics    Clin    Appl.    2009 Nov; 3(11):1314-25. doi: *These authors have contributed equally.

Selected as “Editor’s Choice” in Science (2010) Vol. 327, pp 13.

4. Pallavi  R*,  Acharya  P*,  Chandran  S,  Daily  JP,  Tatu  U.  Chaperone expression profiles correlate with distinct physiological states of Plasmodium falciparum in malaria patients. Malar J. 2010 Aug 19;9: 236. PubMed PMID: 20719001. *These authors have contributed equally.

 5. PesceER,  Acharya P, Tatu U, Nicoll WS, Shonhai A, Hoppe HC, Blatch GL. The Plasmodium falciparum heat shock protein 40, Pfj4, associates with heat shock protein 70 and shows similar heat induction and localisation patterns. Int J Biochem Cell Biol. 2008;40(12):2914-26. Epub 2008 Jul 12.

 

Reviews

1. Acharya P, Kumar R, Tatu U. Chaperoning a cellular upheaval in malaria: heat shock proteins in Plasmodium falciparum. MolBiochemParasitol. 2007 Jun;153(2):85-94.

 

Book Chapters

1. Duffy P E,  Acharya P and Oleinikov A. Cytoadherence of the Malaria Parasite. Encyclopedia of Malaria. Springer Publications. (Accepted 2014)

2. Acharya P, Grover M and Tatu  U.Systems  Biology  of   Malaria  in Encyclopedia of Systems Biology. Springer Publications. 2013.

 

 

1. K. David Raja, M.Sc. Student

 

I did my graduation from Deshbandhu College, DU. I am trying to establish plasma derived nucleic acids as a source of biomarkers in Acute Chronic Liver Failure (ACLF). My current hobby is playing football and reading comics.

 

2. Akshay Munjal, M.Sc. Student

I did my graduation from Deshbandhu College, DU. I am trying to establish appropriate biomarkers for celiac disease using proteomics. My hobby is gardening. My field of interests is protein expression, purification and interaction studies.

 

3. Poornima Gola: Technician

Poornima helps with the general running of the lab and participates in indenting for materials and organizing help for the lab. She likes to read.

 

 

 

 

 

Dr. Rakhee Yadav

Name of Faculty Member: Dr. Rakhee Yadav

Designation: Assistant Professor

Address: Room No. 3027B, Department of Biochemistry, Third Floor, Main Teaching Block, AIIMS, New Delhi-110029

Official Phone: 011-26546514

Email id: यह ईमेल पता spambots से संरक्षित किया जा रहा है. आप जावास्क्रिप्ट यह देखने के सक्षम होना चाहिए.

Short Biography (use template provided)

Academic Details

Degree

Subject

University/ Institution

Year

MBBS

 

Pt. BD Sharma PGIMS, MD University, Rohtak, Haryana

1998-2003

MD

Biochemistry

PGIMS, Rohtak, Pt BD Sharma University of Health Sciences, Rohatk, Haryana

2008-2011

 

Positions held

 

Position

Department

University/ Institution

Dates

Senior Resident

Biochemistry

VMMC and Safdarjung Hospital, New Delhi

08/08/2011- 12/01/2014

Assistant Professor

Biochemistry

SGT Medical College and Hospital; SGT University, Gurgaon (Haryana)

16/01/2014- 09/11/2016

Assistant Professor

Biochemistry

AIIMS, New Delhi

21/11/2016 onwards

 

Notable Awards and Society Memberships

  • Permanent member of AMBI (Association of Medical biochemists of India)
  • Life member of the International Forum of Teachers (IFT) of the UNESCO Chair in Bioethics (Haifa).
  • Trained on Medical Laboratories Quality Management System and Internal Audit as per ISO:15189 at National Institute of Training for Standardization, Bureau of Indian Standards.

Research Focus of the Lab

Presently the area of my interest is to elucidate the underlying cellular mechanisms contributing towards the chronic inflammatory state via the activation of an array of overlapping metabolic and inflammatory cross-talks in the adipose tissue of the obese individuals.

Obesity is a major problem worldwide that increases the risk for a wide range of diseases like diabetes and cardiovascular diseases. The reason why obesity is so closely associated with inflammation and insulin resistance is not completely understood.

An increased Endoplasmic Reticulum (ER) stress in obese individuals may be one of the underlying causes of the inflammatory state and insulin resistance in this metabolically dynamic and complex tissue. So, my current projects cater towards exploring the role of ER stress in obesity induced adipose tissue dysfunction and inflammation

Positions Available (Add a note if you are interested in taking JRF/SRF/Research trainees/MBBS summer fellows)

Applications from motivated, goal-oriented individuals with excellent academic background and experience in molecular biology and immunology having their own fellowships are welcome.

Key publications

  1. Bansal SK, Yadav R. A study of the extended lipid profile including Oxidized LDL, Small dense LDL, Lipoprotein (a) and Apolipoproteinsin the assessment of cardiovascular risk in hypothyroid patients. Journal of Clinical and Diagnostic Research 2016; 10(6): BC04-BC08.
  2. Yadav R, Bansal SK, Karunanand B, Pasha EH, Kumar B, Shastry SP. Association of Thyroid Disorders with the Hormones of Anterior Pituitary in Female Infertility. Annals of Pathology and Laboratory Medicine 2016; 3(4): A340-A346.
  3. Bansal SK, Yadav R, Tanwar R, Chitkara A, Khurana N. Non-Conventional Lipid Parameters for the Assessment of Cardiovascular Risk in Type 2 Diabetes Mellitus Patients in North India. Indian Journal of Health Sciences and Care 2016; 3(2): 49-54.
  4. Ghalaut VS, Yadav R, Dahiya K, Abrol P, Ghalaut PS, Dhankhar R. Levels Of Homocysteine, Folic Acid And Vitamin B12In Patients With Paediatric Acute Lymphoblastic Leukaemia. Australasian Medical Journal 2012; 5(4): 245-246.
  5. Yadav R, Verma SK, Bhartiya JP, Nandkeoliar MK. Changing Trends In Diagnostic Criteria Of Diabetes Mellitus: An Overview. Int  J Pharma Bio Sci 2013 Apr;4(2): 434-444.
  6. Yadav R, Verma SK, Bhartiya JP, Nandkeoliar MK. The Evaluation OfSerum Amylase In The Patients Of Type 2 Diabetes Mellitus, With A Possible Correlation With The Pancreatic Functions. J Clin Diagnostic Res. 2013;7(7):1291-1294.
  7. Yadav R, Bansal SK, Karunanand B, Yadav BK, Arora J. A Comparative Study of Methods of Estimation of LDL-Cholesterol: Direct vs Friedewald’s Formula and Their Impact on Risk Assessment for Coronary Artery Diseases. Indian Journal of Health Sciences and Care 2015;2(1):30-5.

Dr. Ashok Sharma

Address: Room No: 3029, Department of Biochemistry,

                (In-charge: NSC Neurobiochemistry lab)

                AIIMS, New Delhi.

Phone: 011-26593545

Email id: यह ईमेल पता spambots से संरक्षित किया जा रहा है. आप जावास्क्रिप्ट यह देखने के सक्षम होना चाहिए.

यह ईमेल पता spambots से संरक्षित किया जा रहा है. आप जावास्क्रिप्ट यह देखने के सक्षम होना चाहिए.

Skype ID:  ashoksharma35
http://scholar.google.com/citations?user=3ac275cAAAAJ&hl=en
https://www.researchgate.net/profile/Ashok_Sharma12/

Website:  Chromatin & Epigenetics Lab

Areas of Research interest: Cancer EpigeneticDNA MethylationChromatin remodelling & Regulation, Cancer Germline geneEpithelial-Mesenchymal Transition

 

Academic Details:

Degree

Subject

University/ Institution

Year

Ph.D

Biochemistry

All India Institute of Medical Sciences. New Delhi

Sept. 2008

M.Sc

Biochemistry

M.D University, Rohtak, HR

2001

D. Pharmacy

Pharmacy

Pt. B.D.S PGIMS, Rohtak, HR

1999

B.Sc

Medical

D.G College, Gurugram, HR

1997

Advance Certificate courses

Epigenetic Control of Gene Expression

University of Melbourne, Australia

2014

 

Positions held:

Position

Department

University/ Institution

Dates

Assistant Professor

 

Biochemistry

 

All India Institute of Medical Sciences, New Delhi

06/06/2015

DBT-Ramalingaswami Fellow

Dept. of Obstetrics &Gynecology

All India Institute of Medical Sciences, New Delhi

20/04/2015

Postdoctoral Fellow

Eppley Institute for Research in Cancer

University of Nebraska Medical Center (UNMC), Omaha, NE, USA

15/10/2012

Postdoctoral Fellow

Surgical Oncology

Vanderbilt Medical Center, Nashville, TN,  USA

15/10/2008

 

Awards/ Fellowships:

  1. Ramanujan Fellowship Jan., 2015, DST, India
  2. Ramalingaswami Re-entry Fellowship, Nov. 2014, DBT, India
  3. Postdoctoral Travel Award in ASBMB Annual Meeting, held in conjunction with Experimental Biology 2012 in San Diego, CA, USA.
  4. “AIIMS Excellence Award” (2012) for research article
  5. Awarded by GeneCopoeia, Inc in AACR-2011 Conference held at Orlando, Florida, USA.
  6. Senior Research Fellowship Award(ICMR- PHA/BMS/2006), Indian Council of Medical Research (ICMR),
  7. College colour’ for outstanding performance in academics and co-curricular activities during B.Sc. at D. G College, Gurgaon (1997).
  8. College colour’ for outstanding performance in academics and co-curricular activities during B.Sc. at D. G College, Gurgaon (1996).

 

Society Memberships:

  1. Life member of Indian Society of Cell Biology (ISCB) (2016)
  2. Life member of Indian Association for Cancer research (IACR) (2015)
  3. Member of International Epigenetics Society (DNA Methylation Society) (2013-till date)
  4. Associate member of the American Association for Cancer Research (AACR)#247189(2009-till date)
  5. Associate member of The American Society of Clinical Oncology (ASCO) # 205272 (2010-till date)
  6. Associate member of the American Society for Biochemistry and Molecular Biology (ASBMB) #30959 (2012-till date)
  7. Member of Gynecologic Oncology Group, UNMC, Nebraska, USA(2013-till date)
  8. Member of “Association of Scientists of Indian Origin in America, Inc.”(2012-till date)
  9. Member of American Association of Nephrology # 176832(2011-till date)
  10. Member of the American Association of Indian Scientists in cancerResearch (AAISCR)(2012-till date)
    1. Life member of the, The Indian Biophysical Society” (L-549), India (Life member)

Research Focus of the Lab:

Assertive discoveries have been carried out during the past decade to explore the role of epigenetic mechanisms for the regulation of cellular phenotype. This regulation provides the software that packages DNA, modifications in gene expression without changing the DNA sequence of a gene. These are called epigenetic alterations. These alterations include methylation, acetylation, phosphorylation and ubiquitylation of DNA and histone proteins (nucleosomes) as well as chromatin remodelling. “Epigenetics” refers to heritable changes in gene expression patterns without changing the primary DNA sequence. Technological advancements in genome-wide DNA sequencing, RNA sequencing for coding and non-coding expression patterns, DNA methylation and chromatin assay and assessment of all of the above with advance bioinformatics tools are facilitating to delineate the cancer epigenome and permit key insights for developing personalized therapeutics. Our lab basically focuses on cancer epigenetics and we are trying to decipher the epigenome regulation that may lead to development of new epigenetic therapeutic drugs.  The recent discovery of Cancer Testis/Germline (CT/CG) antigen expression in cancer suggests a strong link between gametogenesis and carcinogenesis.CG antigens have the potential to serve as biomarkers for cancer stem cells and potential targets in cancer metastatic processes as well as in the recurrence of cancer after chemotherapy. We are indulging to open the new avenues for novel Cancer Testis/Germline (CT/CG) POTE antigens to be used for cancer immunotherapy for treating gynaecological cancer patients for better reproductive health. POTE (Prostate, Placenta, Ovary, and Testis-expressed) is a recently discovered gene family consisting of 13 autosomal and pericentromeric localized cancer-testis/germline antigen genes. The POTE paralogs gene family encodes proteins with cysteine-rich (CRR) domains, ankyrin repeat motifs and α-helical regions. It is established that epigenetic mechanisms control expression of CT/CG antigens. Epigenetic modulatory agents robustly promote expression of CG antigens, as well as the class-1 histocompatibility complex (MHCI).Thus, we emphasize the possible clinical use of epigenetic modulators to augment the immunotherapeutic potential of POTE family antigens, and how this will ultimately improve strategies for cancer detection and treatment.

 

Towards the mechanistic part, we are working on chromatin dynamics to be applied for answering key questions of alteration in pericentromeric localized gene expression during cancer. POTEs gene family are localized at pericentromeric regions, where duplicated genes are often abundant. Still, it is not clear how POTE paralogs escape pericentromeric repression and become expressed in cancer. Chromatin has a defined 3-D organization inside the nucleus, which guides gene expression. Although this 3D Genome organization is just recently being defined to the molecular levels, there is much to be answered in this area. Our lab is working to correlate the established molecular mechanisms for gene regulation e.g DNA methylation to be linked to the 3D genome organization inside the nucleus. It is known that there are several repressive compartments inside the nucleus where genes are dynamically located as a whole. Therefore what is the relation of epigenetic gene regulation to the gene localization and arrangement inside the nucleus? This is an inevitable question here. One such repressive compartment is nuclear lamina, where the associated sequences, called "Lamina-associated domains (LADs)" and “pericentromeric-associated domains (PADs)”are dynamically placed. We are trying to find factors, responsible for these LADs /PADs formation and regulation to be applied for cancer biology. Altogether this is an entirely new area in the field of cancer biology and studies are coming with new fascinating facts, and we are trying to be part of these strategies, with an ultimate goal to combat cancer. For further detail please visit my website: “Chromatin & Epigenetics Lab

 

List of Lab Members:

  1. Mohit Arora, CSIR-JRF
  2. Dhruv Das, M.Sc Student
  3. TriptiMehra,M.Sc Student
  4. Dr. Arpita Suri, M.D, DNB (Senior Resident)
  5. Dr. Syed Ghazanfar Imam, M.D student
  6. Neeraj Kumar, Lab Attendant

 

Positions Available:

My laboratory is aimed to identify early detection, diagnosis and treatments for gynaecological cancer. The goal of our research program is to explore how epigenetic processes integrate cellular signalling and the metabolic state to influence cell proliferation, differentiation, and adaptation during both normal development and cancer.  We also concentrate to define the epigenomic programs, controlled by DNA methylation, chromatin, and nucleosome positioning, that control the extensive self-renewal properties associated with cancers. This research is providing new insights into cancer pathogenesis and unique opportunities to develop rational approaches for potential use of cancer testis/germline antigen as new personalized therapeutic strategies. Highly motivated and interested fellows and students are encouraged to inquire regarding the availability of research positions.

We are seeking applications from motivated, goal-oriented individuals with excellent academic background and having their own CSIR/DBT/ICMR/BINC/DST fellowships. Candidates with experience in Molecular Biology/Bioinformatics or other multidisciplinary are encouraged to contact and send their CV along with a cover letter at यह ईमेल पता spambots से संरक्षित किया जा रहा है. आप जावास्क्रिप्ट यह देखने के सक्षम होना चाहिए.

 

Key publications:

Google Scholar, (http://scholar.google.com/citations?hl=en&user=3ac275cAAAAJ)

  1. Bhagwan Sharma J, Sneha J, Singh UB, Kumar S, Kumar Roy K, Singh N, Dharmendra SAshokSharma , Sharma E. Effect of Antitubercular Therapy on Endometrial Function in Infertile Women with Female Genital Tuberculosis.Infect Disord Drug Targets. 2016;16(2):101-8.
  2. Smitha R. James#, Carlos Cedeno#, Ashok Sharma, Wa Zhang, James L. Mohler, KunleOdunsi. Elizabeth M. Wilson and Adam R. Karpf. DNA methylation and nucleosome occupancy regulate MAGEA11 expression in human cancer. Epigenetics, 2013. July, 8(8).PMID: 23839233
  3. Ashok Sharma, Moorthy Krishnan Amar B. Singh, PunitaDhawan*. Trichostatin-A Modulates Claudin-1 mRNA Stability Through the Modulation of HuR and Tristetraprolin in Colon Cancer Cells.Carcinogenesis2013 Jul 23.PMID: 23880304
  4. Jillian L. Pope, Ajaz. A. Bhat, Ashok Sharma, Rizwan Ahmad, Mary K. Washington, Robert D. Beauchamp, Amar B. SinghandPunitaDhawan. Claudin-1 Regulates Intestinal Epithelial Homeostasis through the Modulation of Notch Signaling. GUT, 2013 PMID: 23766441
  5. Ashok Sharma*, Singh AB*, Dhawan P. Claudin-1 Expression Confers Resistance to Anoikis in Colon Cancer Cells in an Src-Dependent Manner. Carcinogenesis. 2012. Dec; 33(12): 2538-47. PMID: 22941059
  6. Ajaz Ahmad Bhat, Ashok Sharma, Krishnan M, Mary K Washington, Amar B Singh, PunitaDhawan. "Caudal Homeobox Protein Cdx-2 Cooperates with Wnt pathway to Regulate Claudin-1 Expression in Colon Cancer Cells". PLoS One. 2012; 7(6): e37174.PMID: 22719836
  7. Ashok Sharma*, Singh AB*, Krishnan M, Smith JJ, Beauchamp RD, Dhawan P. Claudin-1 regulates E-cadherin expression through ZEB-1/TCF-8/dEF-1 in Colon Cancer Cells. Gastroenterology, 2011. Dec; 141(6): 2140-53. PMID: 21878201
  8. Akhter, zahid, Ashok Sharma and Moganty R. Rajeswari. Interaction of Adriamycin with a Promoter Region of hmga1 and its inhibitory effect on HMGA1 expression in A431 human squamous carcinoma cell line. MolBiosyst. 2011 Apr 1; 7(4): 1336-46. PMID: 21336378
  9. Krishnan M, Singh AB, Smith JJ, Ashok Sharma, Chen X, Eschrich S, Yeatman TJ, Beauchamp RD, Dhawan P. HDAC inhibitors regulate claudin-1 expression in colon cancer cells through modulation of mRNA stability. Oncogene. 2010 Jan 14; 29(2): 305-12. PMID: 19881542
  10. Ashok Sharma,Ramanjaneyulu A, Ray R, Rajeswari MR. Involvement of High Mobility Group B Proteins in Cisplatin-induced Cytotoxicity in Squamous Cell Carcinoma of Skin. DNA Cell Biol. 2009 Jul; 28(7): 311-318.PMID: 19435426

 

Current Grants:

 

  1. Estimate the Serum Levels of Cancer-Testis Antigen POTE as a Biomarker in Epithelial Ovarian Cancer (2016)- Intramural, AIIMS
  2. Genome-Wide DNA Methylation & Genomic Instability Profiling and Evaluating Its Potential as a Biomarker and Immunotherapeutic Target for Ovarian tumor- DBT, India

 

1. Mohit Arora

 I am currently exploring the dynamics of chromatin organization and its relation to epigenetic remodelling during cancer. I have completed my Master's degree in Biotechnology from Banaras Hindu University in 2015, where I gained understandings of cancer biology and various treatment approaches for cancer. Now in AIIMS under the guidance of Dr. Ashok Sharma, I am exploring some novel tumor antigens and the epigenetic regulation in relation to dynamics of chromatin architecture in cancer.

  

2.  Parisha Singh

 

I have done my Post graduation in Bioinformatics from Banaras Hindu University, 2015. Recently I have joined Dr. Ashok Sharma's lab at AIIMS, New Delhi. I am looking forward to learning the wet lab techniques and applying my bioinformatics tools and skills to find out the expression and function of Cancer Testis Antigens in Ovarian Cancer so that we can diagnose it in future.

 

3. Triptimehra

 

I am currently pursuing M.Sc Biochemistry, AIIMS. I have completed my graduation from Punjab University in Biotechnology. I am exploring epigenetic changes in Ovarian Cancer.

 

4. Dhruv das

 

I am currently pursuing M.Sc in Biochemistry Department, AIIMS. I have completed my graduation in biochemistry from Deshbandhu College, University of Delhi. I had a great interest in the field of cancer biology and epigenetics, therefore, I am exploring ovarian cancer and epigenetic changes in it.

  

5.Dr Arpita Suri

She has completed her MBBS degree from MAMC, New Delhi in 2010. She has done her MD Biochemistry from Lady Harding Medical College in 2014. She worked on the role of cytokines, insulin resistance and TNF-α Polymorphisms in IHD patients as her MD dissertation for which she was awarded the Lord Sreenivasa of Seven Hills Gold Medal for original research in atherosclerosis at ISARCON. She has given poster presentations at various conferences and was also awarded NIMS best poster award at ACBICON, New Delhi. She has also won 2nd prizein the quiz competition at AMBICON,Delhi chapter, New Delhi. She also gave a oral presentation on her thesis work at ICCAD, Italy. She completed her DNB (Biochemistry) in 2015.She is currently pursuing her Senior Residency at AIIMS and working on a research project regarding the role of novel Cancer Testis antigen POTE and its epigenetic regulation in the aetiopathogenesis of epithelial ovarian cancer.

 

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