Primate Implantation Biology Laboratory
Incharge : Prof. J.Sengupta
The Primate Implantation Biology Laboratory at the Department of Physiology, has the following broad objectives
Exploring endometrial receptivity to blastocyst implantation in the primate.
Examining the cellular and molecular process of placentation in the primate
A zona-free blastocyst implants into the receptive stage uterine endometrium. The physiological definition of endometrial receptivity to blastocyst implantation in the primate has undergone evolution with time. Using the rhesus monkey as an experimental model, we have investigated this topic under different paradigms.
We have demonstrated that endometrial receptivity to blastocyst implantation is primarily induced by luteal phase progesterone. This knowledge has an important bearing on the possible endocrine interventions in physiognomonic and pathognomonic domains for blastocyst implantation. Thus, this knowledge may be used for improvising strategies for inhibiting blastocyst implantation, as well as, for assisting the subset of women suffering from inadequate endometrial receptivity leading to infertility.Although the new knowledge about endocrinology of blastocyst implantation process in primates from our studies appears promising, it does not address the paracrine interactions among various endometrial cells under progesterone dominance. Also, the nature of interaction between endometrium and implanting blastocyst appears important in the process of successful implantation and placentation. The studies conducted in our laboratories have indicated some important and interesting points of revelation in this regard. Recently, systems biology approach using genomic and post-genomic paradigms appears promising in exploring hither-to enigmatic 'vacuum response', and 'network based sufficiency and purposedness' in the process of blastocyst implantation and placentation. We, at the Primate Implantation Laboratory of Department of Physiology at the All India Institute of Medical Sciences, are engaged in the task of deciphering the functional network of a wide range of expression arrays displayed by receptive stage primate endometrium in time and space contexts using human and non-human samples.
Our studies have been supported by various organizations that include the World Health Organization, the Rockefeller Foundation, the Contraceptive Research & Development (CONRAD), All India Institute of Medical Sciences (AIIMS), Council of Science and Industrial Research (CSIR), Indian Council of Medical Research (ICMR), Department of Biotechnology (DBT), and Department of Science and Technology (DST), Government of India.
List of Publications in the year 2006 to till date
Accessing modern contraceptive technologies in India
Indian J Physiol Pharmacol. 2006;50(4):327-340.
Ghosh D, Najwa AR, Sengupta J
In vitro action of leukemia inhibitory factor (LIF) on mid-secretory stage endometrial stromal cells collected from hormone-simulated, ovariectomized monkey and maintained in three-dimensional primary culture.
Indian J Physiol Pharmacol. 2006 Oct-Dec;50(4):355-66.
Sengupta J, lalitkumar PG, Najwa AR and Ghosh D
Monoclonal anti-leukemia inhibitory factor antibody inhibits blastocyst implantation in the rhesus monkey.
Contraception. 2006 Nov;74(5):419-25.
Sengupta J, Lalitkumar PGL, Najwa AR, Charnock-jones DS, Evans AL, Sharkey AM, Smith SK and Ghosh D
Immunoneutralization of vascular endothelial growth factor inhibits pregnancy establishment in the rhesus monkey (Macaca mulatta).
Reproduction 2007: 133: 1-14
Lalitkumar PGL, Sengupta J, Ghosh D (2005) Endometrial tumour necrosis factor a (TNFa) is a likely mediator of early luteal phase mifepristone mediated negative effector action on preimplantation embryo. Reproduction (In Press).
Ghosh D, Sengupta J (2005) Target oriented anti-implantation approaches for pregnancy interception: Experiences in the rhesus monkey model. Contraception (In Press).
Lamba P, Kar M, Sengupta J, Ghosh D (2005) Effect of (Ala8,13,18)-magainin II amide on human trophoblast cells in vitro. Indian J Physiol Pharmacol 49: 27-38.
Kar M, Sengupta J, Kumar S, Bhargava VL, Ghosh D (2004) Immunohistochemical localization of macrophage CD68+, HLA-DR+, L1+ and CD44+ subsets in uterine endometrium during different phases of menstrual cycle. Indian J Physiol Pharmacol 48: 293-303.
Ghosh D, Bell SC, Sengupta J (2004) Immunohistological localization of insulin-like growth factor binding protein-1 in primary implantation sites and trauma-induced deciduomal tissues of the rhesus monkey. Placenta 25: 197-207.
Ghosh D, Sengupta J (2004) Endometrial and paracrine correlates of endometrial receptivity to blastocyst implantation in the human. Indian J Physiol Pharmacol 48: 6-30.
Sengupta J, Dhawan L, Lalitkumar PGL, Ghosh D (2003) A multiparametric study of the action of mifepristone used in emergency contraception using the rhesus monkey as a primate model. Contraception 68: 453-469.
Sengupta J, Dhawan L, Ghosh D (2003) Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey. Cytokine 24: 277-285.
Kar M, Lalitkumar PGL, Dhawan L, Sengupta J, Ghosh D (2002) Effect of vaginally administered fumagillin on immunohistochemical distribution of leukemia inhibitory factor, interleukin 6, transforming growth factor b and vascular endothelial growth factor in implantation stage endometrium of the rhesus monkey. Indian J Physiol Pharmacol 46: 25-35.
Sengupta J, Ghosh D (2002) Blastocyst-endometrium interaction at implantation in the rhesus monkey. J Reprod Immunol 53: 227-239.
Ghosh D, Dhawan L, Lalitkumar PG, Wong V, Rosario JF, Hendrickx AG, Lasley BL, Overstreet JW, Sengupta J (2001) Effect of vaginally administered (Ala8,13,18)-magainin II amide on the morphology of implantation stage endometrium in the rhesus monkey (Macaca mulatta). Contraception 63: 335-342.
Venkataraman L, Upadhyay S, Lalitkumar PG, Sengupta J, Ghosh D (2001) Morphological and functional characteristics of rabbit uterine epithelial cells grown on free floating collagen gel. Indian J Physiol Pharmacol 45: 161-171.
Ghosh D, Lalitkumar PG, Wong V, Dhawan L, Rosario JF, Hendrickx AG, Lasley BL, Overstreet JW, Sengupta J (2001) Effect of vaginally administered fumagillin on the morphology of implantation stage endometrium in the rhesus monkey. Contraception 63: 95-102.
Dhara S, Lalitkumar PG, Sengupta J, Ghosh D (2001) Immunohistochemical localization of insulin-like growth factors I and II at the primary implantation site in the Rhesus monkey. Mol Hum Reprod 7: 365-371.