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अखिल भारतीय आयुर्विज्ञान संस्थान, नई दिल्ली
All India Institute Of Medical Sciences, New Delhi

Bacteriology, Serology & S.T.D. Section

List of investigations offered in the Bacteriology, Serology & S.T.D. Section

  1.        Direct Examination of sample received
    1. Gram’s stain
    2. Albert stain
    3. Methylene blue
    4. Hanging drop : Motility for Vibrio cholera
    5. Wet mount preparation for Urine
  2. Specimen processing (Culture) for Blood, Bone marrow, CSF, Pus, Bile, Urine, Respiratory sample (Sputum, BAL, ET aspirate, Lung aspirate), Sterile Body fluids, Fecal specimen, Catheter tips of the central lines, Throat/nasal swabs, Other Swabs, Drain, Aspirate, Biopsy, Cervical discharge, Urethral discharge, Semen etc
  3. Processing and identification for the Gram Positive Cocci
  4. Processing and identification of the Gram Negative Bacilli (lactose fermenter)
  5. Processing and identification for Gram Negative Bacilli (Nonlactose fermenter)
  6. Identification of aerobic spore forming bacilli
  7. Bacterial Identification by MALDI–TOF (Matrix Assisted Laser Desorption/Ionization – Time of Flight
  8. Detection of antimicrobial susceptibility by CLSI method
    1. Disc diffusion ( Kirby Bauer)
    2. MIC by E test
    3. Broth dilution assay
    4. Detection of special resistance mechanism
      1.                                                               i.      MRSA
      2.                                                             ii.      VRE
      3.                                                           iii.      ESBL
  9. Antigen detection test  for S. pneumoniae, H. influenzae, N. meningitidis, E. coli, S. agalactiae)
  10.  Typing methods:
    1. Biotyping for species identification
    2. Serotyping for Vibrio cholerae, Salmonella species, Beta haemolytic Streptococci, Shigella species
  11. Serology Laboratory:
    1. Widal test
    2. Antistreptolysin O
    3. VDRL
    4. TPHA
  12. Sexually Transmitted Diseases (STD) laboratory: Specimen from the STD clinics and Gynaecology Department are received for screening for the reproductive tract infection (RTI) or the bacterial STDs. The direct smears for Bacterial Vaginosis or vaginitis are screened. Microscopy and cultures for N. gonorrhoeae and the antibiotic resistance monitoring is undertaken. Besides the semen specimen are cultured in case any bacterial infection are suspected. On request PCR for N. gonorrhoeae and C. trachomatis for uro-genital samples is also available. Culture for T. vaginalis is also available.
  13. Hospital Infection Control Laboratory: This section is involved with the surveillance of health care associated infections in the Institute. The number of health care associated infections including BSI (CLABSI), UTI (CAUTI), SSI, VAP, Hand hygiene compliance is recorded and infection rates determined. Environmental sampling is done to monitor effective decontamination and disinfecting procedures carried out in the hospital.

Central Sterile Supply Department (CSSD) autoclaves are monitored by Biological indicators to check for the functioning of the autoclaves. The sterility of the fluids or pre-sterile articles purchased is tested on the request of the Medical Supply Stores. Monitoring of antimicrobial resistance is done by keeping a record of the resistance pattern of the bacteria causing infections in our hospital. The change in the infection rates and outbreaks are identified and the concerned unit informed in time to take timely control measures. Surveillance of the staff to detect any carrier state in outbreaks is undertaken.

This section also involves with carrying out teaching activities and sensitization of all new health care staff to hospital infection activities.

  1. Water Bacteriology: This section tests the bacteriological quality of water in the Institute hospital and residential areas to monitor proper treatment of water being supplied to the Institute
  2. External quality assurance Scheme (EQAS)

Contact Us

All India Institute of Medical Sciences

Ansari Nagar, New Delhi - 110029

Board Number : +91-11-26588500 / 26588700

Fax : +91-11-26588663 / 26588641

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