Tissue (Biological) processing for TEM
Tissue (Biological) processing for SEM
Elemental analysis by EDAX
1. Transmission Electron Microscopes
a) Morgagni 268D (Fei Electron Optics)
b) TECNAI 200 Kv TEM (Fei, Electron Optics)
Both equipped with digital imaging and 35 mm photography system
2. Scanning Electron Microscope
Leo 435 VP
- Works in low and high vacuum mode
- Equipped with digital imaging and 35 mm photography system
3. Energy-dispersive x-ray microanalysis (EDAX) attached to the SEM
- For elemental analysis of biological as well as non-biological samples.
4. Ultramicrotomes: For cutting ultrathin sections for TEM
a. Reichert-Jung Ultracut-E
b. Leica Ultracut – UCT
c. Leica UC6
5. Critical Point Dryer: For drying wet biological samples for SEM.
a. Jumbo critical point dryer (Bio-Rad)
b. EMS-850 Critical point dryer (Emitech)
6. Sputter Coater Unit: For metal coating of dry biological samples viewed under high vacuum SEM.
a. Balzer Union SCD 020
b. Agar Sputter Coater
Information for the users
The Facility is open from 9.30 A.M. till 5.15 P.M. on all working days from Monday to Friday and from 9.30 A.M. to 1.00 P.M. on Saturdays. Prior booking is necessary for the users (in-house as well as outsiders) to work in the SAIF. The sample collection time is 11.00 A.M. to 1.00 P.M. on all working days except Saturday.
Payment has to be made in two parts:
1. Payment at the time of sample submission
On the basis of number of samples submitted and type of processing of the specimens, one can calculate the amount from the rate-list (refer rate-list) and the same has to be deposited along with the samples.
2. Payment after microscope viewing and image recording
The final payment will be done after calculating the TEM viewing time and number of images recorded (refer rate-list) by the user.
The payment should be made by bank draft in favour of “AIIMS (Scheme Fund), New Delhi”. Personal cheques are not accepted. Payment can also be made in cash in the Research Section of AIIMS.
SAIF Organizes regular training programs, workshops and symposia on Electron Microscopy for manpower development. These are as follows:
A National training programs
(a) For technical personnel
This training programme is conducted during January/February every alternate year and is of 2-week duration. Training is imparted in the maintenance and operation of electron microscopes and other ancillary equipment. The trainees are also given training on sample preparation, ultrathin sectioning and grid preparation for TEM and sample preparation, critical point drying and sputter coating for SEM.
(b) For scientific investigators
This training programme is conducted during October/November each year and is of 2-week duration. The first half is devoted to basic course and the second half is for the advance techniques. In the basic course, theoretical and practical aspects of electron microscopy, such as principles of operation, use and maintenance of electron microscopes and specimen preparation are included. In the advanced course, training is imparted on special techniques like immuno-electron microscopy, stereology and image analysis.
(c) Summer training
A six-week extensive hands-on training on sample preparation and operation of both SEM and TEM is held every year tentatively from 15th May for a period of six weeks. The objective of the training program is to enhance the skills of technical support staff for an electron microscope Laboratory.
Procedure for applications for training programs
A Committee scrutinizes the applications and the selected candidates are intimated to join the training.
The application should be sent to:
Officer-in-Charge, E.M. Facility, Department of Anatomy, AIIMS, New Delhi 110 029.
The aim of SAIF-AIIMS is to strive for excellence and ensure maximal utilization of the national resource.
The facility is committed to
Provide assistance to research on electron microscopy by investigators,
Provide expertise for the use of equipment, interpretation and evaluation of ultrastructural data,
Develop new techniques in ultrastructural research to facilitate scientists working in the front-line areas of research and
Organize regular training programs, workshops and symposia on Electron Microscopy for manpower development.
|Name of Faculty||Designation||E-mail ID|
Taposh Kumar Das
Tapas Chandra Nag
Sandeep Arya, Technical Officer
Chanda Panwar, Lab Technician
Anurag Singh, Lab Technician
Madan Mohan Sharma, Lab Technician
Meherban Singh, Lab Technician
Raj Girish Mishra, Lab Attendant II
Non-technical staff :-
Manjeet Kapoor, Private Secretary
Sudhir Srivastava, Upper Division Clerk (Stores)
The Electron Microscope (EM) laboratory was established in the year 1971 in the Department of Anatomy of All India Institute of Medical Science (AIIMS). Recognizing the contribution of the laboratory and the need for the ultrastructural investigations, the Department of Science and Technology (DST), Government of India established a Regional Electron Microscope facility in 1983 at AIIMS under its RSIC/SIF program. This center is presently known as Sophisticated Analytical Instrument Facility (SAIF) – AIIMS, New Delhi, under the SAIF Program of DST.