The Molecular Biology Laboratory in the Department of Physiology is equipped with Bio-Rad Phosphoimager and Real Time PCR. Array-based technologies allow rapid and simultaneous analysis of the expression of large numbers of genes across a wide variety of tissues and cells. cDNA-based expression array technique is amenable for measuring the abundance of RNA for targeted genes in biological sample. Phosphoimager can be used for imaging of screens exposed to hybridized membrane, or DNA stained gel, protein stained gel, chemifluorescence, chemiluminescence for perfoming densitometric analyses.
Image of hybridized membrane showing mRNA expression profile of
luteal phase endometrium.
Real time PCR based validation of quantitative
ratio shift in expression array using TaqMan
based mutiplex FRET
*Contact Details:- Prof. D.Ghosh, Department of Physiology, AIIMS, New Delhi-29. Room No. 2005. Tel No. 26594625.