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अखिल भारतीय आयुर्विज्ञान संस्थान, नई दिल्ली
All India Institute Of Medical Sciences, New Delhi

Lab Services

Lab Services

I HAEMOGRAM LAB

Haemogram including Haemoglobin, TLC,DLC, Platelet count, MCV, MCH, RDW, Haematocrit, peripheral smear, reticulocyte count.

  • Bone marrow morphology from bone marrow aspirate and bone marrow touch smear-Giemsa stain
  • Bone marrow biopsy haematoxylin eosin stain
  • Cytochemistry & immunophenotyping
II ANEMIA LAB
  • Haemoglobin A2 and F estimation
  • High performance liquid chromatography (HPLC) for detection of abnormal haemoglobins & their quantitation including HbF and A2
  • Hb electrophoriesis for HbF & A2 and Haemoglobinopathy
  • Sickling test
  • Serum iron studies- S. iron , TIBC, % saturation
  • Coomb’s test both direct and indirect manual and by gel technology
  • Ham’s test and sucrose lysis test for PNH
  • Heinz bodies /HbH inclusion
  • G6PD screening test
  • Osmotic fragility test
  • Plasma haemoglobin
  • Urine haemosiderin
  • Serum protein electrophoresis for plasma cell dyscrasia
  • Cryoglobulin
III COAGULATION LAB
  • Bleeding time
  • Prothrombin time
  • Partial thromboplastin time with Kaolin
  • Thrombin time
  • Fibrinogen
  • Euglobulin clot lysis time
  • Clot solubility test
  • D-Dimer
  • Factor assay for – Factor V, VIII,IX, X and XIII
  • Inhibitor studies for Factor V, VIII,IX, X and XIII
  • PF3 availability test with ADP
  • Total PF3
  • Platelet function test
  • INR for monitoring of oral anticoagulant therapy
  • Lupus anticoagulant
  • thrombophilia work up [ AT II, Protein C , Protein S, APC-R] Proglob C
  • Ristocetin Co-F
  • VWD antigen
  • Beta 2 glycoprotein

MOLECULAR TESTS

Thrombophilic mutations
  • Prothrombin P20210 G®A mutation
  • MTHFR C6 77T gene polymorphism
  • Factor V leiden mutation
Haemophilia
  • Intron 22 inversion
  • Intron 1 inversion
  • Carrier detection and prenatal diagnosis by linkage analysis
Glanzmann’s Thrombasthenia
  • GP IIb//IIIa western blotting
  • GP IIb//IIIa flow cytometry
VWD
  • Multimeric analysis by western blotting
  • Carrier detection by linkage analysis
Thalassemia
  • Alpha thalassemia mutation- 3.7 and 4.2 deletions

Leukemia

RT-PCR for
  • CML (BCR-ABL) Philadelphia
  • APML t(15;17)
  • AML t(8;21), Inv 16
  • ALL t(9;22), t(1;19), t(12;21), t (4;11)
CYTOCHEMISTRY
  • Sudan black (SB)
  • Myeloperoxidase (MPO)
  • Leukemia alkaline phosphatase (LAP)
  • PAS,
  • NSE and NSE-F (Non specific esterase)
  • Acid phosphatase
  • Double esterase
Real Time PCR

Test conducted by the department

IMMUNO PHENOTYPING FOR LEUKEMIAS

Flow cytometry laboratory has been carrying out immunophenotpying of acute, chronic leukemias, lymhoproliferative disorders, hereditary platelet disorder, red blood cell defects, CD34 stem cell count for hematopoietic stem cell transplantation.

BLOOD COUNTS

It is stabilized whole blood specimens. The laboratories performing test on auto-analyzers should report all the parameters listed in the results sheet. The manually operating labs may reports as many parameters as they feel feasible.

RETICULOCYTES COUNT

Please assess the retic count (%) on the slide provided. Counting the red cells correctly can be helped by inserting into the eyepiece or cardboard diaphragm with a small hole in the center to reduced the optical field so that not more than 30-35 R.B.Cs should be viewed in a field

PERIPHERAL BLOOD SMEAR

The blood films are stained with jenner – giemsa stain. Please report DLC and brieflycomment on R.B.C. morphology i.e. report five noticeable/relevant features observed . Only first five will be included in your assessment. Reporting of diagnosis is not essential, if you do decide to report diagnosis, please mention only one possibility. Wrong diagnosis will results in poor performance score. The slip of clinical summary is inserted inside the slide mailer

ISHTM – AIIMS EXTERNAL QUALITY ASSURANCE PROGRAMME, HAEMATOLOGY DEPTT, AIIMS, NEW DELHI

Email - This email address is being protected from spambots. You need JavaScript enabled to view it.
AIIMS CODE NO._____________ DIST NO_________________
  1. Please send your results by email - This email address is being protected from spambots. You need JavaScript enabled to view it. typing email ID, code & dist. Number, results, full address of your lab. and feed back comments on a single sheet only. No separate covering letter is acceptable. Scanned copy of result sheet is not acceptable
  2. You are likely to receive the specimens by 7th date of March, June, September and December. In case, you don’t receive the specimens by these dates due to any transit problem, please inform us by email immediately so another set of specimens could be sent. No any complaint for non- receipt of specimens will be entertained after above mentioned dates. Never forget to quote your code no.
  3. We issue acknowledgement on receipt of EQAP results to the email senders. If you don’t receive acknowledgment for the same within 2-3 days of sending your results, it should be assumed that your results have not reached us and you should take follow-up steps by confirming it on email.
  4. The email senders of results get acknowledgement , many vital informations and early delivery of evaluation reports. The non- email senders of results lose all these facilities.
  5. Your results must reach us by last working day of March, June, Sept. & Dec. The results received after these dates will not be included for evaluation.
  6. The Annual Participation Fees, effective from January to December to be paid in advance in the form of a demand draft drawn in favour of ISHTM (Indian Society of Hematology & Transfusion Medicine) It should payable in New Delhi. Address it to ‘Prof. Incharge, Quality Assurance Progamme, Room No. 156, Haematology Deptt., 1st Floor, IRCH Building, AIIMS, New Delhi- 110029’. No multicity cheque or any other type of cheque acceptable.

    The fees should be paid at the following rates:

    (i) Govt Owend/ ISHTM Members’ Personal Labs only- Rs.1000/-

    (ii) All other Private Labs – Rs.1500/-

  7. We try our best to supply the specimens free from microbial contamination, HIV and Hbs Ag., however, they should be treated as if from patients and care taken in their handling and disposal.
  8. The specimens have the capability to withstand ambient temperature during the transit period without any deterioration; however, the cool pack is placed in the specimens box as an additional precaution.
  9. The blood specimens should be stored at 4°C immediately after receiving and processed as soon as possible.
  10. No any query will be attended on telephone. You may convey your message on email - This email address is being protected from spambots. You need JavaScript enabled to view it. . Only for inquiring about receipt of results, you may contact on Ph. No. 9968113832 or 011- 26593642

To become a member of the Quality Control Programme the following form may be filled and sent to us.

APPLICATION FORM

To send report please download form

ISHTM- AIIMS EQAP- RESULTS SHEET

Email form…………………………………… E-mail to This email address is being protected from spambots. You need JavaScript enabled to view it.

Code no…………………………….. Dist no…………………………………………..

Note:(1) you must perform each test including retic count twice and report two value in the prescribed column

(2) Please send your results by email This email address is being protected from spambots. You need JavaScript enabled to view it. typing email ID, code and dist. Number, results, full address of your lab. And feedback comments on a single sheet No. separate covering letter is acceptable Scanned copy of result sheet

is not acceptable

Test ParametersSample No.First ResultsSecond Results
WBC – 103 µl. - - -
RBC – 106 µl. - - -
Hb- g/dl - - -
HCT- % - - -
MCV- fl. - - -
MCH – Pg - - -
MCHC- g/dl. - - -
Platelet - 103 µl. - - -
Retic Count % - - -

REPORT OF BLOOD FILM NO. 3

No. of cells counted_______________________ Polymorphs %________________

NRBC/ 100 WBCs ________________________Lymphocytes % _____________

Blast % ________________________ Eosinophis % _________________

Promyelocytes %_ ______________________ Monocytes % __________________

Myelocytes % ________________________ Basophils %___________________

Meta Mylecytes %________________________ Stab % _______________________

Others % ________________________

RBC Morphology_____________________________________________________

_____________________________________________________

Diagnosis (Optional)___________________________________________________

Full Address of Your Lab:-------------------------------------------------------------------_

Date of Receipt of Specimens………………………………………………………… _

Quality of specimens : No.1…………………..No.2……………….No.3………….._

Suggestion/ Usefulness of programme………………………………………………………. _

……………………………………………………………………………………………….. _

You must quote your code no., full address of your lab. & dist in your results sheet and other correspondence.

Contact Us

All India Institute of Medical Sciences

Ansari Nagar, New Delhi - 110029

Board Number : +91-11-26588500 / 26588700

Fax : +91-11-26588663 / 26588641

AIIMS Exam Section Helpline numbers ( Click Here)

Important E-mail Addresses at AIIMS

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