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अखिल भारतीय आयुर्विज्ञान संस्थान, नई दिल्ली
All India Institute Of Medical Sciences, New Delhi

Dr. Kalpana Luthra

 

 

 

 Address: Room No-4001, 4th Floor,

                 Department of Biochemistry,

                 Convergence Block,

                 All India Institute of Medical Sciences,

                 New Delhi-110029

Phone: 011-26549242

Email Id This email address is being protected from spambots. You need JavaScript enabled to view it.

Academic Details:

  • PhD in Biochemistry in the year 1994, All India Institute of Medical Sciences, New Delhi.

  • Masters in Science (M.Sc in Biochemistry) in the year 1987, SIES College, Mumbai

Dr. Kalpana Luthra is a Professor in the Department of Biochemistry, All India Institute of Medical Sciences (AIIMS), New Delhi, India. She completed her PhD at AIIMS in 1994 and joined as faculty at AIIMS in 1998. She was awarded the Fogarty fellowship in 2002 and availed training in HIV-1 antibody related work at New York University.

 

Research Focus of the lab

HIV Immunology: Coevolution of virus and antibody responses

HIV-1 is a complex virus with a remarkable ability to protect itself from our intricate network of immune system. This is achieved by masking itself from the immune recognition by acquiring the host proteins or by escaping the humoral response by virtue of its extraordinary ability to mutate. This leads to generation of diverse strains of HIV-1 virus, making the treatment and prevention a complicated process. More than 90% of the HIV-1 infections in India are caused by clade C viruses. Our lab focuses on studying various aspects of HIV-1 infection which includes:

  • Characterizing the antibody  responses against the circulating virus in infected donors, particularly infected infants and pediatric patients.

  • Generation and characterization of human anti-HIV-1 recombinant monoclonal antibodies as potential therapeutic reagents against HIV-1.

  • To study the coevolution of virus and antibody responses during natural infection and map neutralization determinants on the HIV-1C viruses circulating in infant and adult infected individuals to identify clade C specific epitopes for immunogen design.

  • Currently, our lab is in the process of generating native-like, soluble HIV-1 envelope immunogens and characterizing their potential as vaccine candidates in rabbits.

 

Studies on pediatric HIV-1 infection  

HIV-1C primary isolates generated in our lab from select infected children showed resistance to neutralization by majority of the known second generation bNAbs (Makhdoomi et al, Virology 2016). We followed up antiretroviral naïve chronically infected children and mapped their plasma for presence of bnAbs (Makhdoomi et al. J Gen Virol. 2017 Jul;98(7):1879-189) and identified a pair of identical twins AIIMS_329 and AIIMS_330. The plasma from both donors showed the evolvement of bnAbs targeting common epitopes in the V2 and V3 regions of the envelope, suggesting bnAb development in these twins may perhaps be determined by specific sequences in the shared virus that can guide development of immunogens aimed at eliciting V2 and V3 bNAbs. Characterization of the neutralization sensitive and resistant viruses coevolving with bNAbs in the contemporaneous AIIMS_330 plasma provides information towards understanding viral alterations that may have contributed to the development of resistance to bnAbs. (Mishra N. J Virol https://doi.org/10.1128/JVI.00654-19).  

Our work on circulating HIV-1 in perinatally infected infants defined viral factors associated with early bnAb responses, supportive of polyvalent vaccination approaches capable of inducing bnAbs against HIV-1 (Mishra N. Nat.Commun.,2020) 11:4409 https://doi.org/10.1038/s41467-020-18225-x). As in case of the monozygotic twins, we identified that the antibody response in infants primarily targets the V2 region of HIV-1, making it an attractive target for immunogen design to elicit antibodies.  

Using the high throughput technology of HIV-1 specific single B cell sorting, we isolated a bNAb AIIMS-P01 from a pediatric elite neutralizer (Kumar et al, JVI, 2019). This mAb has shown 67% breadth at a potency of 0.5 µg/ml, and shows good neutralizing activity against Indian clade C viruses and needs to be tested in future for in vivo protection against HIV-1 infection.

 

SARS-CoV-2

During the COVID-19 pandemic, neutralizing antibodies have been shown to be a critical feature of recovered patients. Given the unique nature of HIV-1 bnAbs and their ability to recognize and/or accommodate viral glycans, we reasoned that the glycan shield of SARS-CoV-2 spike protein can be targeted by HIV-1 specific bnAbs. We showed that HIV-1 specific monoclonal antibodies as well as polyclonal plasma antibodies cross-react and neutralize SARS-CoV-2. (Mishra N. PLoS Pathog 2021. 17(9): e1009958https://doi.org/10.1371/journal.ppat.1009958.  

Publications (Select publications since last 5 years)

  1. Ray MD, Deo SVS, Luthra K, Mathur S, Anand P, Kumar R, Sharma S, Kahol S, Wahid A. Molecular linking of HIPEC (Hyperthermic Intraperitoneal Chemotherapy) and T-regulatory cells (FOXp3) and Th-17 (RORɣ) in advanced epithelial ovarian cancer. Clin Immunol Commun. 2022 Dec 1;2:62–8.doi.org/10.1016/j.clicom.2022.03.005

  2. Mishra N, Kumar S, Singh S, Bansal T, Jain N, Saluja S, Kumar R , Bhattacharyya S , Jayanth Palanichamy J, Mir RA,  Sinha S , Luthra K. (Sept 2021) Cross-neutralization of SARS-CoV-2 by HIV-1 specific broadly neutralizing antibodies and polyclonal plasma. PLoS Pathog. 2021 Sep 24;17(9):e1009958. doi: 10.1371/journal.ppat.1009958. eCollection 2021 Sep.PMID: 34559854

  3. Singh V, Nambirajan A, Malik PS, Thulkar S, Pandey RM, Luthra K, Arava S, Ray R, Mohan A, Jain D. Spectrum of uncommon and compound epidermal growth factor receptor mutations in non-small-cell lung carcinomas with treatment response and outcome analysis: A study from India. Lung Cancer. 2020 Nov;149:53-60. doi: 10.1016/j.lungcan.2020.07.038. Epub 2020 Sep 13. PMID: 32971387

  4. Mishra N, Sharma S, Dobhal A, Kumar S, Chawla H, Singh R, Makhdoomi MA, Das BK, Lodha R, Kabra SK, Luthra K. Broadly neutralizing plasma antibodies effective against autologous circulating viruses in infants with multivariant HIV-1 infection.  Nat Commun. 2020 Sep 2;11(1):4409. Nat Commun. 2020; 11: 4409. Published online 2020 Sep 2. doi: 10.1038/s41467-020-18225-x PMCID:  PMC7468291

  5. Mohan A, Ansari A, Masroor M, Saxena A, Pandey RM, Upadhyay A, Luthra K, Khilnani GC, Jain D, Kumar R, Guleria R. Measurement of Serum EGFR mRNA Expression is a Reliable Predictor of Treatment Response and Survival Outcomes in Non- Small Cell Lung Cancer.  Asian Pac J Cancer Prev. 2020 Nov 1;21(11):3153-3163. doi: 10.31557/ APJCP. 2020. 21.11.3153. PMID: 33247670

  6. Mishra N, Sharma S, Dobhal A, Kumar S, Chawla H, Singh R, Das BK, Kabra SK, Lodha R, Luthra K. A Rare Mutation in an Infant-Derived HIV-1 Envelope Glycoprotein Alters Interprotomer Stability and Susceptibility to Broadly Neutralizing Antibodies Targeting the Trimer Apex.  J Virol. 2020 Sep 15;94(19):e00814-20. doi: 10.1128/JVI.00814-20. Print 2020 Sep 15. PMID: 32669335

  7. Kumar S, Batra H, Singh S, Chawla H, Singh R, Katpara S, Hussain AW, Das BK, Lodha R, Kabra SK, Luthra K. Effect of combination antiretroviral therapy on human immunodeficiency virus 1 specific antibody responses in subtype-C infected children.  J Gen Virol 2020 Dec;101(12):1289-1299. doi: 10.1099/jgv.0.001480. Epub 2020 Sep 11

  8. Sahu AK, Aggarwal P, Ekka M, Nayer J, Bhoi S, Kumar A, Luthra K.Top of FormAssessing the serum chymase level as an early predictor of dengue severity. J Med Virol. 2020 Aug 28. doi: 10.1002/jmv.26468. Online ahead of print. PMID: 32857465

  9. Sharma V, Makhdoomi M, Singh L, Kumar P, Khan N, Singh S, Verma HN, Luthra K, Sarkar S, Kumar D.Trehalose limits opportunistic mycobacterial survival during HIV co-infection by reversing HIV-mediated autophagy block. Autophagy. 2020 Feb 20:1-20. doi: 10.1080/15548627.2020.1725374

  10. Panda S, Tiwari A, Luthra K, Sharma SK, Singh A.Association of Fok1 VDR polymorphism with Vitamin D and its associated molecules in pulmonary tuberculosis patients and their household contacts. Sci Rep. 2019 Oct 24;9(1):15251. doi: 10.1038/s41598-019-51803-8

  11. Kumar R, Parray HA, Shrivastava T, Sinha S, Luthra K. Phage display antibody libraries: A robust approach for generation of recombinant human monoclonal antibodies. Int J Biol Macromol. 2019 Aug 15;135:907-918. doi: 10.1016/j.ijbiomac.2019.06.006. Epub 2019 Jun 3

  12. Panda S, Tiwari A, Luthra K, Sharma SK, Singh A. Status of vitamin D and the associated host factors in pulmonary tuberculosis patients and their household contacts: A cross sectional study. J Steroid Biochem Mol Biol. 2019 Oct;193:105419. doi: 10.1016/j.jsbmb.2019.105419.

  13. Singh V, Guleria P, Malik PS, Mohan A, Thulkar S, Pandey RM, Luthra K, Arava S, Ray R, Jain D.Epidermal growth factor receptor (EGFR), KRAS, and BRAF mutations in lung adenocarcinomas: A study from India. Curr Probl Cancer. 2019 Oct;43(5):391-401 doi: 10.1016/j.currproblcancer.2018.12.003.

  14. Nitesh Mishra, Madhav Mohata, Rajeev Narang, R. Lakshmy , Anjali Hazarika, R. M. Pandey , Nibhriti Das  and Kalpana Luthra. Altered Expression of Complement Regulatory Proteins CD35, CD46, CD55, and CD59 on Leukocyte Subsets in Individuals Suffering From Coronary Artery Disease, Frontiers in Immunology, 2019 Aug 29;10:2072 doi: 10.3389/fimmu.2019.02072

  15. Mishra N, Makhdoomi MA, Sharma S, Kumar S, Dobhal A, Kumar D, Chawla H, Singh R, Kanga U, Das BK, Lodha R, Kabra SK, Luthra K Viral characteristics associated with maintenance of elite neutralizing activity in chronically HIV-1 clade C infected monozygotic pediatric twins. J Virol. 2019 Aug 13;93(17). :e00654-19 doi: 10.1128/JVI.00654-19.

  16. Mochan S, Dhingra MK, Gupta SK, Saxena S, Arora P, Yadav V, Rani N, Luthra K, Dwivedi S, Bhatla N, Dhingra R. Status of VEGF in preeclampsia and its effect on endoplasmic reticulum stress in placental trophoblast cells. Eur J Obstet Gynecol Reprod Biol X. 2019 Jun 21;4:100070. doi: 10.1016/j.eurox.2019.100070

  17. Jayasundar R, Ghatak S, Makhdoomi MA, Luthra K, Singh A, Velpandian T.Challenges in integrating component level technology and system level information from Ayurveda: Insights from NMR phytometabolomics and anti-HIV potential of select Ayurvedic medicinal plants. J Ayurveda Integr Med. 2019 Apr - Jun;10(2):94-101: doi: 10.1016/j.jaim.2017.06.002.

  18. Kumar S, Panda H, Makhdoomi MA, Mishra N, Safdari HA, Chawla H, Aggarwal H, Reddy ES, Lodha R, Kumar Kabra S, Chandele A, Dutta S, Luthra K An HIV-1 broadly neutralizing antibody from a clade C infected pediatric elite neutralizer potently neutralizes the contemporaneous and autologous evolving viruses. J Virol. 2019 Feb 5;93(4). e01495-18. doi: 10.1128/JVI.01495-18.

  19. Kumar R, Kumari R, Khan L, Sankhyan A, Parray HA, Tiwari A, Wig N, Sinha S, Luthra K.Isolation and Characterization of Cross-Neutralizing Human Anti-V3 Single-Chain Variable Fragments (scFvs) Against HIV-1 from an Antigen Preselected Phage Library. Appl Biochem Biotechnol. 2019 Mar;187(3):1011-1027. 10.1007/s12010-018-2862-8

  20. Mishra N, Mohata M, Aggarwal H, Chaudhary O, Das BK, Sinha S, Hazarika A, Luthra K. Expression of complement receptor 3 (CR3) and regulatory protein CD46 on dendritic cells of antiretroviral naïve and treated HIV-1 infected individuals: Correlation with immune activation status. Mol Immunol. 2018 Apr;96:83-87 doi: 10.1016/j.molimm.2018.02.011.

  21. Aggarwal H, Khan L, Chaudhary O, Kumar S, Makhdoomi MA, Singh R, Sharma K, Mishra N, Lodha R, Srinivas M, Das BK, Kabra SK, Luthra K. Alterations in B Cell Compartment Correlate with Poor Neutralization Response and Disease Progression in HIV-1 Infected Children.Front Immunol. 2017 Dec 1;8:1697. doi: 10.3389/fimmu.2017.01697

  22. Kumar S, Kumar R, Khan L, Makhdoomi MA, Thiruvengadam R, Mohata M, Agarwal M, Lodha R, Kabra SK, Sinha S, Luthra K.CD4-Binding Site Directed Cross-Neutralizing scFv Monoclonals from HIV-1 Subtype C Infected Indian Children. Front Immunol. 2017 Nov 15;8:1568

  23. Makhdoomi MA, Khan L, Kumar S, Aggarwal H, Singh R, Lodha R, Singla M, Das BK, Kabra SK, Luthra K. Evolution of cross-neutralizing antibodies and mapping epitope specificity in plasma of chronic HIV-1-infected antiretroviral therapy-naïve children from India. J Gen Virol. 2017 Jul;98(7): 1879-1891. doi: 10.1099/jgv.0.000824.

  24. Ali A, Kumar S, Kakaria VK, Mohan A, Luthra K, Upadhyay AD, Guleria R. Detection of Promoter DNA Methylation of APC, DAPK, and GSTP1 Genes in tissue Biopsy and Matched Serum of Advanced-Stage Lung Cancer Patients. Cancer Invest. 2017 Jul 3;35(6):423-430. doi: 10.1080/07357907.2017.1309547

  25. Mohan A, Poulose R, Gupta T, Luthra K, Pandey RM, Madan K, Hadda V, Guleria R. Impact of chemotherapy on symptom profile, oxidant-antioxidant balance and nutritional status in non-small cell Lung Cancer. Lung India. 2017 Jul-Aug;34(4):336-340. doi: 10.4103/0970-2113.209230.

  26. Khan L, Kumar R, Thiruvengadam R, Parray HA, Makhdoomi MA, Kumar S, Aggarwal H, Mohata M, Hussain AW, Das R, Varadarajan R, Bhattacharya J, Vajpayee M, Murugavel KG, Solomon S, Sinha S, Luthra K.Cross-neutralizing anti-HIV-1 human single chain variable fragments(scFvs) against CD4 binding site and N332 glycan identified from a recombinant phage library.Sci Rep. 2017 Mar 23;7:45163. doi: 10.1038/srep45163

  27. Mohan A, Arora S, Uniyal A, Poulose R, Luthra K, Pandey RM, Guleria R. Evaluation of plasma leptin, tumor necrosis factor-α, and prealbumin as prognostic biomarkers during clinical recovery from acute exacerbations of chronic obstructive pulmonary disease. Lung India. 2017 Jan-Feb;34(1):3-8. doi: 10.4103/0970-2113.197101.

  28. Makhdoomi MA, Singh D, Nair Pananghat A, Lodha R, Kabra SK, Luthra K. Neutralization resistant HIV-1 primary isolates from antiretroviral naïve chronically infected children in India.Virology. 2016 Sep 16;499:105-113. doi: 10.1016/j.virol.2016.09.011.

 

Chapters in Books

  • Chapter on “Indian Scientist major contribution in the field for Nobel Prize” in “Nobel Dreams of India” Editors: Dr Archana Sharma, Dr. Sweta Vijaykrishnan. 2020

  • Muzamil A. Makhdoomi, Lubina Khan, Sanjeev Kumar, Heena Aggarwal, Ravinder Singh, Rakesh Lodha, Mohit Singla. Bimal K Das, Sushil K. Kabra and Kalpana Luthra. Title of the Chapter: “Cross-neutralizing antibodies envolve in plasma of chronic HIV-1 infected ART naïve children (long term non-progressors) from India”, (Editors: Satish Kumar Gupta and Nirmal Kumar Lohiya), Name of the Book: “Molecular Medicine Bench to Beside and Beyond” 2018, page 253-260, Publisher: Indian society for the study of reproduction and fertility, University of Rajasthan.

  • Chapter on The Thyroid Glandin ‘Textbook of Biochemistry, Biotechnology, Molecular Biology and Allied Medicine’ (Editors: Dr. G.P. Talwar, Dr. SeyedE. Hasnain and Dr. ShivKumarSarin. 2016

  • ‘Fat metabolism and its regulation’. Published in “Concepts of Biochemistry”. Ed. Dr. L.M.Srivastava, CBS Publications, pp. 189-248, 2004.

  • Two chapters on Centrifugation and Electrophoresis under Laboratory Instrumentation in “Essentials of Biochemistry”.  LM Srivastava (Editor), CBS Publishers, New Delhi, India. Chapters 3.1 & 3.5 Page 11-18 & 32-37, 2002.

  • Chapter on DNA Fingerprinting for class XI-XII ‘Textbook in Biology (supplementary material) published by the Central Board of Secondary Education (CBSE) in December 2000. Chapter on “Basic concept of Clinical Biochemistry” in e book “Clinical Biochemistry”, 18th September 2006. (Revised 5th May 2008). http://nsdl.niscair.res.in/jspui/bitstream/123456789/691/1/ClinicalBiochem_Concepts.pdf

 

Patents   

INDIAN:

  1. Invention Title: “Neutralizing human anti-V3 monoclonal antibody generated from HIV-1 Clade C infected Indian patient” Authors: Dr. Kalpana Luthra, Dr. Raiees Andrabi, Dr. Naveet Wig, Dr. Ashutosh Biswas, Patent Application No: 1882/DEL/2012. The above patent was advertised and granted on 26 February 2014 (Patent number: ZA Patent No. 2013/04490).

  2. Invention Title: “A BROADLY NEUTRALIZING ANTIBODY” Authors: Dr. Kalpana Luthra, Sanjeev Kumar, Dr Rakesh Lodha: Indian Provisional Patent Application No.: 201811021940, 12th June, 2018,LIPC Ref: 55/PA/AJ/CA/2018.

  3. Invention Title: “Neutralizing human anti-V3 monoclonal antibody generated from HIV-1 Clade C infected Indian patient” Authors: Dr. Kalpana Luthra, Dr. Raiees Andrabi, Dr. Naveet Wig, Dr. Ashutosh Biswas, Patent Application No: 1882/DEL/2012. The above patent was advertised and granted on 26 February 2014 (Patent number: ZA Patent No. 2013/04490). 

  4. Invention Title Novel engineered HIV-1 Subtype-C envelope glycoproteinProf. Kalpana Luthra, Mr. Swarandeep Singh, Dr. Sanjeev Kumar, Mr. Nitesh Mishra, Ms. Shaifali Sharma, Dr. Muzamil Ashraf Makhdoomi, Dr. Rakesh Lodha. Applied for professional patenting through AIIMS, New Delhi, in December, 2021. (Reference Number: F:1-132/2021/P.Cell, Application Number: 202211004612)

  

Honors/Awards/Achievements  

  • AIIMS Excellence Research Award 2019 in Basic Research (1stPrize)

  • Elected as “Women in Science of Antiviral Research Society and was awarded the same on 24th October 2017 by Antiviral Research Society at the National Seminar on Recent advances in Antiviral Drug Design against Emerging and Re-emerging viral diseases held in Kalasalingam University, Krishnakoil, Tamil Nadu

  • AIIMS Excellence Research Award 2017 in Basic Research (3rdPrize)

  • AIIMS Excellence Award 2013 in Basic Sciences (2nd Prize)

Shaifali Sharma, Ph.D. Scholar 

 

Ms. Shaifali Sharma is a Ph.D. Scholar in Prof. Luthra’s Laboratory since February 2020. As a part of her thesis, she is generating HIV-1 envelope immunogen which binds to germline version of known anti-HIV-1 broadly neutralizing antibodies from early HIV-1 infected donors.

She earned her Master’s degree in Medical Biochemistry from AIIMS, New Delhi in 2019. As a part of her M.Sc. thesis dissertation, she studied the HIV-1 envelope glycoprotein by generating pseudoviruses from a chronically infected long term non-progressor, in order to identify envelope features of the virus circulating in patient. After completing her M.Sc., she continued being a part the Lab as a Junior Research Fellow (joined in October 2019) and later registered for Ph.D. in February 2020.

Previous Research Work

  1. Generation and characterization of HIV-1 subtype-C based trimeric immunogens from infected children. (2019- present)  

  2. Generation and characterization of pseudoviruses from children with perinatally acquired HIV-1 infection (2017-2021)  

  3. Generation and characterization of pseudoviruses from an adult HIV-1 infected Long term non-progressor (LTNP) for potential subtype C based immunogen. (2017-2019)  

  4. Dissemination of antibiotic resistance among airborne bacteria and its public health implications. (2015-2016)

 

Publications

  1. Ray MD, Deo SVS, Luthra K, Mathur S, Anand P, Kumar R, Sharma S, Kahol S, Wahid A. Molecular linking of HIPEC (Hyperthermic Intraperitoneal Chemotherapy) and T-regulatory cells (FOXp3) and Th-17 (RORɣ) in advanced epithelial ovarian cancer. Clin Immunol Commun. 2022 Dec 1;2:62–8.doi.org/10.1016/j.clicom.2022.03.005

  2. Mishra N, Sharma S, Dobhal A, Kumar S, Chawla H, Singh R, Makhdoomi MA, Das BK, Lodha R, Kabra SK, Luthra K. Broadly neutralizing plasma antibodies effective against autologous circulating viruses in infants with multivariant HIV-1 infection. Nat Commun. 2020 Sep 2;11(1):4409. doi: 10.1038/s41467-020-18225-x.  

  3. Mishra N, Sharma S, Dobhal A, Kumar S, Chawla H, Singh R, Das BK, Kabra SK, Lodha R, Luthra K. A rare mutation in an infant derived HIV-1 envelope glycoprotein alters interprotomer stability and susceptibility to broadly neutralizing antibodies targeting the trimer apex. J Virol. 2020 Jul 15:JVI.00814-20. doi: 10.1128/JVI.00814-20. Online ahead of print.  

  4. Mishra N, Sharma S, Dobhal A, Luthra K. A versatile high throughput strategy for cloning the env gene of HIV-1. bioRxiv. 2020.04.01.020735; doi: 10.1101/2020.04.010.020735.  

  5. Mishra N, Makhdoomi MA, Sharma S, Kumar S, Dobhal A, Kumar D, Chawla H, Singh R, Kanga U, Das BK, Lodha R, Kabra SK, Luthra K. Viral Characteristics Associated with Maintenance of Elite Neutralizing Activity in Chronically HIV-1 Clade C-Infected Monozygotic Pediatric Twins. J Virol. 2019 Aug 13;93(17). pii: e00654-19. doi: 10.1128/JVI.00654-19. Print 2019 Sep 1.

 

Workshops/Conferences/Symposium attended/ Awards and Distinctions

  1. Qualified and secured 67th All India rank in Joint CSIR-UGC Junior Research Fellowship and Eligibility for Lectureship (JRF-NET) exam, June 2019.

  2. Qualified and secured 434th All India Rank in GATE-2019 (Graduate Aptitude Test in Engineering) for Life Sciences, February 2019.

  3. 1st prize for M.Sc. Poster titled “Generation and characterization of pseudoviruses from an adult HIV-1 infected long term non progressor for potential subtype C based immunogen design” at AIIMS first annual research day event, March 2019.

  4. World Immunology Day Symposium on “Gazing into the Horizon of Immune System”, May 2018.

  5. International Conference on Strategies for Environmental Protection and Management (ICSEPM-2016), December 2016.

  6. Workshop on Intellectual property rights: culmination of research, September 2016.

Association with professional bodies

Life member of Indian Immunology society since April 2018.

Patents filed

  1. Invention titled “Development of CRISPR based detection assay for clade C Human Immunodeficiency Virus-1” Prof. Kalpana Luthra, Ms. Anjli Guar, Mr. Harsh Bhakhri, Mr. Nitesh Mishra, Ms. Shaifali Sharma, Ms. Tanu Bansal, Prof. Subrata Sinha, Prof. Rakesh Lodha, Applied for patenting through AIIMS, New Delhi, in September 2022.

  2. Invention titledNovel engineered HIV-1 Subtype-C envelope glycoproteinProf. Kalpana Luthra, Mr. Swarandeep Singh, Dr. Sanjeev Kumar, Mr. Nitesh Mishra, Ms. Shaifali Sharma, Dr. Muzamil Ashraf Makhdoomi, Dr. Rakesh Lodha. Applied for professional patenting through AIIMS, New Delhi, in December, 2021. (Reference Number: F:1-132/2021/P.Cell, Application Number: 202211004612)

 

 Swarandeep Singh, PhD Scholar:

 

Mr. Swarandeep Singh is a fourth year PhD student working in HIV-1 Immunology Laboratory under the chief supervision of Professor Kalpana Luthra. As part of his PhD thesis, he is working on “Generation and Characterization of HIV-1 subtype- C native like envelope trimeric immunogen from a pediatric elite neutralizer”. He is interested to generate a recombinant HIV-1 envelope trimer glycoprotein invitro, that can mimic the native envelope present on HIV-1 virion and further testing its ability to generate a immunogenic response in small animal models. Parallelly he is working on another project in which he is working on development and characterization of HIV-1 specific lineage antibodies isolated by affinity maturation from pediatric elite neutralizers.

Earlier he did his Masters in Microbiology from Gurukul Kangri University. His master’s dissertation was conducted from Division of Microbiology, Central Drug Research Institute where he studied the role of deletions of promoters in mycobacterium smegmatis.

 

Conferences/Poster or Oral Presentations/Workshops/Awards:

  1. Received Global Health Award for  Keystone Symposia on Next Generation HIV Vaccines & Therapies - C2

  2. Attended SPR seminar and AKTA Pure UNICORN workshop conducted by Cytivia

  3. Participated in 48th Annual Conference of Indian Immunology Society 2022

  4. Attended symposia on Mathematics in Biomedical Sciences organized by Indian National Science Academy INSA

  5. Qualified and secured 53th All India rank for JRF (NET) in Joint CSIR-UGC Junior Research Fellowship and Eligibility for Lectureship (JRF-NET) exam, June 2018.

  6. Qualified and secured 65th All India rank for Lectureship in Joint CSIR-UGC Junior Research Fellowship and Eligibility for Lectureship (JRF-NET) exam, Dec 2018.

  7. Qualified and secured 45th All India rank for Lectureship in Joint CSIR-UGC Junior Research Fellowship and Eligibility for Lectureship (JRF-NET) exam, June 2017.

  8. Qualified and secured 1868th All India rank for Life Sciences in Graduate Aptitude Test in Engineering GATE 2018.

  9. Participated in workshop on Microbiological Laboratory Techniques in Medical Microbiology conducted in Department of Botany & Microbiology, Gurukul Kangri University Haridwar

  10. Participated in workshop on Advanced Microbiological Techniques in Pharmaceutical Industries conducted in Department of Botany & Microbiology, Gurukul Kangri University Haridwar

 

Publications:  

Effect of combination antiretroviral therapy on human immunodeficiency virus 1 specific antibody responses in subtype-C infected children. Kumar S, Batra H, Singh S, Chawla H, Singh R, Katpara S, Hussain AW, Das BK, Lodha R, Kabra SK, Luthra K. J Gen Virol. 2020 Dec;101(12):1289-1299. doi: 10.1099/jgv.0.001480. Epub 2020 Sep 11. PMID: 32915123

Non-neutralizing SARS CoV-2 directed polyclonal antibodies demonstrate cross-reactivity with the HA glycans of influenza virus. Murugavelu P, Perween R, Shrivastava T, Singh V, Ahmad Parray H, Singh S, Chiranjivi AK, Thiruvengadam R, Singh S, Yadav N, Jakhar K, Sonar S, Mani S, Bhattacharyya S, Sharma C, Vishwakarma P, Khatri R, Kumar Panchal A, Das S, Ahmed S, Samal S, Kshetrapal P, Bhatnagar S, Luthra K, Kumar R.  Int Immunopharmacol. 2021 Oct;99:108020. doi: 10.1016/j.intimp.2021.108020. Epub 2021 Jul 29. PMID: 34426117; PMCID: PMC8318684.

The SARS CoV-2 spike directed non-neutralizing polyclonal antibodies cross-react with Human immunodeficiency virus (HIV-1) gp41 Perween R, PraveenKumar M, Shrivastava T, Parray HA, Singh V, Singh S, Chiranjivi A, Jakhar K, Sonar S, Tiwari M, Reema, Panchal AK, Sharma C, Rathore DK, Ahamed S, Samal S, Mani S, Bhattacharyya S, Das S, Luthra K, Kumar R. Int Immunopharmacol. 2021 Dec;101(Pt B):108187. doi: 10.1016/j.intimp.2021.108187. Epub 2021 Sep 28. PMID: 34649114; PMCID: PMC8479463.

Cross-neutralization of SARS-CoV-2 by HIV-1 specific broadly neutralizing antibodies and polyclonal plasma Mishra N, Kumar S, Singh S, Bansal T, Jain N, Saluja S, Kumar R, Bhattacharyya S, Palanichamy JK, Mir RA, Sinha S, Luthra K.. PLoS Pathog. 2021 Sep 24;17(9):e1009958. doi: 10.1371/journal.ppat.1009958. PMID: 34559854; PMCID: PMC8494312.  

Recognition determinants of broad and potent HIV-1 neutralization by an affinity matured antibody from a pediatric elite-neutralizer  Kumar S*, Singh S*,Chatterjee A, Bajpai P, Sharma S, Katpara S, , Lodha R, Luthra K. (under Preparation) * Equal Contribution

  

Dr. Sanket Katpara

 

Dr. Sanket Katpara is a second year PhD student and a senior resident at the department of biochemistry working under the chief supervision of Professor Kalpana Luthra in the HIV immunology lab. As part of his PhD thesis, he is working on “Generation and Characterization of recombinant anti-HIV-1 human monoclonal antibodies from HIV-1 infected individuals”. He is interested in generation of monoclonal antibodies that can target different epitopes on the HIV-1 envelope which can be used as a combinatorial therapy for the treatment of drug resistant HIV-1 infected individuals.

Earlier he has also completed his M.D. under the supervision of Dr Kalpana Luthra at the Department of Biochemistry, AIIMS.

Conferences/Poster or Oral Presentations/Workshops/Awards:

  • Poster presentation at the annual international science event BIOZEST 2019 at the South Asian University, New Delhi.

  • Qualified DNB (Biochemistry) exit exam 2021.

 

Antara Malik, PhD scholar:

 

Ms. Antara Malik is a fourth year PhD student currently working in the lab of Dr. Kalpana Luthra. Primarily, her thesis work is aimed at understanding the status of the immune responses and the role of macrophages and T cells  in the immunopathology of Chronic Pancreatitis. The second part of her thesis work involves the elucidation of metabolic alterations associated with the progression of Chronic pancreatitis.

She completed her Masters in Biotechnology from Jamia Millia Islamia and graduated in Biochemistry (Hons.) from University of Delhi. She has previously worked as a Junior Research Fellow in NIPGR (National Institute of Plant Genome Research) and IARI (Indian Agricultural Research Institute) before joining as a PhD student in the lab. 

 

Conferences/Workshops/Achievements:

  1. Qualified National Eligibility Test (NET) exam conducted by Indian Council of Medical Research (ICMR).

  2. Attended a symposium on “Inflammation” organized by THSTI on 14th July 2022.

  3. Attended a symposium on “World Immunology Day” organized by Dept of Biochemistry, AIIMS on 29th April 2022.

  4. Attended a webinar on Single Cell Sequencing: From Single-cell RNA-seq to Spatial Transcriptomics organized by Novogene on 06th November 2020.

  5. Participated in the Immunoinformatics workshop conducted by Decode Life from 23rd-24th September 2020.

  6. Attended an online workshop on flow cytometry organized by Flow Cytometry solutions from 08-13th August 2020.


Harsh Bhakhri, PhD Scholar:

 

Mr. Harsh Bhakhri is a first year PhD student (Dec 2021-present) currently working on the characterization of the B cell receptor repertoire in HIV-1 subtype C infected individuals and also in generation of human anti-HIV-1 antibodies from infected individuals. He is also trying to assess the plasma antibodies neutralization potential against SARS-COV2 in different infected individuals.

He has also completed his M.Sc. in Biochemistry from AIIMS in 2021. His masters dissertation thesis work was to the role of M6A writer METTL3 and readers (IGF2BP1/3) in hematopoietic development and leukemogenesis. He has done his graduation in B.Sc. (Hons) Biochemistry from Sri Venkateswara College, University of Delhi in 2019.

 

 Anjli Gaur, M. Sc. Student

 

 

Ms. Anjli Gaur is a final year M.Sc. student currently working in the lab of Dr. Kalpana Luthra. She joined the lab in June 2021 for her M.Sc. Thesis dissertation. Her work is focused on “Development of CRISPR based detection assay for clade C Human Immunodeficiency Virus-1”. Our immediate goal is to determine the HIV-1 Clade C viral nucleic acid detection(qualitatively)using the CRISPR/Cas system. the long-term goal is to quantitatively determine the viral load.   

She completed her graduation in B.Sc. (Hons.) Biochemistry from Institute of Home Economics, University of Delhi in 2019.

Conferences/Poster or Oral Presentations/Workshops/Awards:

  • Patent filed for work entitled “Development of CRISPR based detection assay for clade C Human Immunodeficiency Virus-1”.

  • Attended the workshop on “NEXT GENERATION GENOME SEQUENCING”

  • She is also National level Taekwondo Champion.

 

Muneeb Pervez, M.Sc. student

 

 Mr. Muneeb Pervez is the 2nd year M.Sc Student in the department currently working under Dr Kalpana Luthra in the HIV immunology Lab. He joined the lab in the October 2021 and is currently working on his M.Sc. thesis titled “Expression and characterization of an HIV-1 Clade C envelope trimer as a potential trimer towards immunogen design”.

He completed his B.Sc (Hons.) in Biochemistry from the department of Biochemistry, Aligarh Muslim University (A.M.U.).

Conferences/Workshops/Awards:

  • Qualified Graduate Aptitude Test in Engineering GATE in Life sciences, 2021.

  • Qualified Graduate Aptitude Test in Engineering GATE in Biotechnology, 2021.  

 

Ajeesh T. Gangadharan , Junior Administrative Assistant

 

Mr. Ajeesh T. Gangadharan joined Dr.K Luthra’s Lab as a Data Entry Operator in a research project on 1st October, 2005. He completed his B. Com from MG University, Kerala in 2002 & Computer Diploma From NIIT, New Delhi in 2004. After the completion of 15 years of service in various research projects, in September 2021 he joined the same lab as a Junior Administrative Assistant. His nature of work includes entering and systematic maintenance of patient data in Excel sheet, to contact and sent written communications for getting quotations for chemicals, preparing supply orders, processing bills, maintaining Accounts of the project, maintaining stock register, compilation of the project reports etc.

 

Mr. Abdul Wahid Hussain, Lab Technician

 

  Mr. Abdul Wahid Hussain is working as a Lab Technician in Dr. Kalpana Luthra’s lab in a research project since January, 2010. He is an expert phlebotomist. His nature of works are cell culture (mammalian and bacterial) media preparation, Buffers preparation etc., PCR, DNA isolation, Patient sample collection, PBMCs isolation, Protein purification, Biological substance distribution to collaborators, Procurement/ordering of chemicals and lab instruments maintenance. He has completed his M.Sc.MLT from NIMS university Jaipur, Rajasthan in year 2020.

Publications:

 

  1. Ray MD, Deo SVS, Luthra K, Mathur S, Anand P, Kumar R, Sharma S, Kahol S, Wahid A. Molecular linking of HIPEC (Hyperthermic Intraperitoneal Chemotherapy) and T-regulatory cells (FOXp3) and Th-17 (RORɣ) in advanced epithelial ovarian cancer. Clin Immunol Commun. 2022 Dec 1;2:62–8.doi.org/10.1016/j.clicom.2022.03.005

  2. Kumar S, Batra H, Singh S, Chawla H, Singh R, Katpara S, Hussain AW, Das BK, Lodha R, Kabra SK, Luthra K. Effect of combination antiretroviral therapy on human immunodeficiency virus 1 specific antibody responses in subtype-C infected children. J Gen Virol. 2020 Dec;101(12):1289-1299. doi: 10.1099/jgv.0.001480. Epub 2020 Sep 11. PMID: 32915123.

  3. Khan L, Kumar R, Thiruvengadam R , parray HA , makhdoomi MA, Kumar S , Aggarwal H, Mohata M , Hussain A W, Das R, Varadarajan R, Bhattacharya J, Vajpayee M, Murugavel KG, Solomon S, Sinha S, Luthra K. Cross- neutralization anti-HIV-1 human single chain variable fragment(scFvs) against CD4 binding site and N332 glycan identified form a recombinant page library. Sci Rep.2017 Mar23;7:45163

 

Karishma Kiran, Lab Technician:

 Ms. Karishma Kiran is working as a Lab Technician in Prof. Luthra’s lab in the Chronic Pancreatitis research project since March, 2022. She is a Diploma holder in Medical Lab Technology from Asian Hospital, Faridabad. Her nature of work includes patient sample collection, PBMC isolation and buffer preparation. Currently, she is in the process of learning PCR, protein purification, DNA isolation, ELISA and flow cytometry. She has previously worked in Max, Multispeciality Hospital, New Delhi where she gained operational knowledge of instruments like Immuno chemistry Analyzer :DXC800 , Blood Culture System :Back Alert 3D480 from Biomerieux, and performed H&E staining, special stain in histopathology, Frozen staining, sure Path Test, Tissue Processing, ABO Blood grouping, Cross Matching, ICT, DCT, Component Preparation and Donor screening.

Suraj Kumar, Lab Technician:

  

 Mr. Suraj Kumar joined Prof. Luthra’s lab in the Chronic Pancreatitis research project as a lab technician in June 2022. He completed his DMLT from DPMI (Delhi Paramedical Management of Institute) in the year 2021. He is an expert phlebotomist and a quick learner. His job duties include patient sample collection, maintaining records of patient clinical data files, PBMC isolation, cell culture media and buffer preparation. Currently, he is in the process of learning primary cell culture, magnetic cell sorting, flow cytometry and data acquisition. He is pursuing BSC (Hons) through distance learning programme from Indira Gandhi National Open University (IGNOU), New Delhi. He has previously worked in Sir Ganga Ram Hospital, New Delhi.

 

Anil Kumar, Masalchi/Bearer Gd.II  

 Mr. Anil Kumar previously worked as a Lab Attendant in Prof. Luthra’s lab in a research project since May, 2003. He was responsible for smooth functioning and maintenance of the lab, which included packing of tips, autoclaving and sterilization of plastic/glasswares, washing of equipment and cleaning of culture lab. He has completed his 12th standard Delhi in 2013. He is employed as Masalchi/Bearer Gd.II from January 2020.

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