Departments 

1.  Mr. P. Jayaraj (2007 onwards)

2.  Miss Sheetal Chauhan (2009 onwards)

This section provides diagnostic services to the Centre which include Clinical pathology, Histopathology and Cytopathology.  It performs on an average 36,000 investigations per year.  In addition  teaching and training programmes in ocular pathology are also conducted  for other medical colleges and hospitals in India.

    Research work on orbital, intraocular tumors & eyelid tumors is being done. The department organized  Ocular pathology workshop under the Indian Association of Ocular Pathologist on 25^th March, 2006.

 A.  Services provided by the ocular pathology section.

 (I)     Histopathology & Frozen section :

      Diagnostic histopathology of ocular specimens received from operation theatre.  In addition frozen section guided excision of lid tumors and frozen section for various histochemical stains is also done.  

(II)   Cytopathology:

a)     Impression Cytology:  Done for diagnosis  of  dry eye,  

corneal ,  conjunctival neoplasias,ulcerative eye lid lesions. 

          b)  FNAC (Fine needle aspiration cytology):  Being done for

 pre-operative diagnosis of palpable orbital, eyelid lesion   

 and enlarged lymph node.

 (III)  Clinical Pathology:

a)      Blood investigations are done with the help of Auto analyzer.

Hemogram –

TLC-Total Leukocyte count.

DLC-Differential Leukocyte count.

ESR- Erythrocyte Sedimentation Rate.

Platelet count.

Peripheral smear. 

b)      Urine analysis – Routine examination including urine for-

         Protein 

         Sugar

         Microscopy.

c)     Sample collection for all investigations at various Dr.  R. P.  

        Centre laboratories.  

(IV)   Immunohistochemistry:   

  Diagnostic and research purposes. 

(V)    Molecular Pathology Laboratory

·     As a part of the Department of Ocular Pathology, this section which was formally inaugurated in March 2009 undertakes tests based on various molecular biology tools including  polymerase chain reaction, MSP, ISH for research purposes.

·     The research is presently related to cancer biology of various ocular malignancies.

·     This section also provides training to undergraduates, post graduate students in basic molecular biology techniques and immunohistochemistry. 

Museum:

Ocular Pathology Museum was started by Prof. Ratnakar in  1976.  Interesting cases of lid, orbital lesion, intra ocular tumor are still being  preserved for teaching purposes.

B.  Research work: 

(a)   Extramural Research Projects: 

1.    ICMR project on “Diagnosis of ocular and orbital round cell tumours in children by Immunohistochemistry” (1989-91). 

2.    ICMR project on “Immunophenotypic characterization of ocular lymphoproliferative disorders” (1998-2001). 

3.    ICMR project on “Prognostic markers in Retinoblastoma – An immunohistochemical study”  (Collaborative with Pediatric Oncology and IRCH.) (2002-05). 

4.    ICMR – “Detection of HPV genome in malignant ocular lesions” (2008-ongoing)  

(b)  Research projects funded by All India Institute of Medical Sciences (AIIMS) 

1.    “Human papilloma virus in conjunctival neoplasias”    (2000-01).  

2.    “Immunohistochemical detection of HSV-1 in non- specific keratitis” (2003-04). 

3.  “P53 expression in sebaceous gland carcinoma of the eye lid- An immunohistochemical study” (2004-05). 

4.    “Retinoblastoma protein  (pRB) expression in human eyes and ocular tumors(2005-06). 

5.    “Immunophenotype characterization of small lymphocytic lymphomas of the eye.  (May-2007).” 

6.    “p53 and mdm2 expression in orbital Rhabdomyosarcoma of childhood and IHC.”  

7.    Identification of Human Papilloma Virus types in malignant ocular tumours (May 2009)

8.    Immunohistochemical detection of cancer stem cell markers in retinoblastoma. ((may 2009)

(c)   Collaborative Research Projects: 

1.  An open labeled, prospective study to evaluate the efficacy  and safety  of ReliNethra (an autologous bio-engineered composite limbal  epithelial graft). (Reliance)-completed 

2.  Efficacy and safety of Restasis in management of dry eye  July 2006 (completed). 

3.  Culture of Limbal stem cells for ocular surface reconstruction in stem cell deficiency diseases (DBT)-2006.(completed) 

4.  Assesment of Safety and efficacy of  Relinethre ^TM – EAU (an antilogous bioengineered conjunctiva) in the treatment of conjunctival disorders (2008 onwards).

5.  IH and molecular studies on WntB-Catenin signaling pathway in  sebaceous cell carcinoma of the eye lid. (Ph.D.Thesis – March 2007 onwards). 

6.  Isolation, Expansion & characterization of corneal limbal epithelial  cells.  (Ph.D. Thesis – February 2006 onwards)  

7.  Identification and characterization of differentially expressed gene in human retinoblastoma-(PhD Thesis –March 2006 onwards).  

8.    Expression of cell cycle regulatory proteins in Ocular Surface Squamous Neoplasia (PhD Thesis February 2009)

Research Publications: 

1.  Kashyap S, Pushker N, Meel R, Sen S, Bajaj MS et al  Orbital 

     schwannoma with cystic degeneration. Clin and Exp

     Ophthalmolo 2009;37(3): 293-8.

2.    Sen S, Sharma A, Panda A. Indian J Ophthalmol. 2007,

     55:361-3. Immunohistochemical localization of human 

     papilloma virus in conjunctival neoplasias: A retrospective 

     study. 

3.    Kashyap S, Sen S, Sharma MC, Betharia SM, Bajaj MS:  

     Alveolar soft part sarcoma of the orbit: report of three cases.  

     Can J Ophthalmology 2004 ; 39 : 552-556.

4.    Sen S, Mahindrakar A, Betharia SM, Bajaj MS, Kashyap S,

     Ghose S : Pleomorphic adenomas of the lacrimal gland:a

     clinicipathological analysis : Clin Exp Ophthalmol 2004 ; 32:

     523-525. 

5.    Sen S, Venkatesh P, Moolchand.Primary Intraocular Hydatid  

     cyst with Glaucoma. J Pediatr Ophthalmol.Strabismus. 2003;

     40 (5): 312-3.

6.    Sen S,  Kashyap S, Singh UB, Nagasuresh V, Chand M, Garg

     SP Intraocular tuberculosis mimicking retinoblastoma-A case

     report. Diagnostic Cytopathology 2003; 28: 107-109.

7.    Sen S, Kashyap S, S.M.Betharia SM: Primary Orbital  

     Plasmacytoma-A case report Orbit 2003; 22: 317-319.

8.    Sen S, Kashyap S, Thanikachalam S, Betharia SM:  Primary

     Primitive Neuroectodermal tumour of the orbit. Report of a

     case. J Pediatr Ophthalmol Strabismus 2002; 39: 242-244.

9.    Kashyap S, Sen S, Sharma MC, Sethi A. Diagnostic intraocular aspiration cytology of choroidal melanoma. Diagn. Cytopathol. 2002: 26: 389- 391.    

10. Kashyap S,Sen S,Betharia SM,Dade VK; Mesenchymal  

      chondrosarcoma of the orbit a clinicopathological study Orbit

      2001:20;63-68.

11. Kashyap S.kapila k,Verma K;Cytologic spectrum seen in fine  

      needle aspirates from Thyroglossal Cysts:J Cytol 2001:18;21-

      24.

12. Kashyap S: Intoacoular fine needle aspiration biopsy in     

      Retinoblastoma.DOS Times 2001:7;26.

13. Kashyap S,Kapila K,Kumar N,Geeta G,Rath GK,Verma

      K;Nucleolar organizer regions and morphologic subtypes of

      squamous cell carcinoma of squamous cell carcinoma of

      cervicx..Ind J Pathol Microbiol 1998;41:303-308.