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This
section provides diagnostic services to the Centre which include Clinical
pathology, Histopathology
and Cytopathology. It performs on an average 36,000
investigations per year. In addition teaching
and training programmes in ocular pathology are also conducted
for other medical colleges and hospitals in India.
Research work on orbital, intraocular tumors & eyelid tumors is
being done. The department organized Ocular pathology workshop
under the Indian Association of Ocular Pathologist on 25th
March, 2006.
A.
Services provided by the ocular pathology section.
(I)
Histopathology
& Frozen section :
Diagnostic histopathology of ocular specimens received from
operation theatre. In
addition frozen section guided excision of lid tumors and frozen
section for various histochemical stains is also done.
(II) Cytopathology:
a)
Impression
Cytology: Done for diagnosis of dry eye,
corneal
, conjunctival neoplasias,ulcerative eye lid lesions.
b)
FNAC (Fine needle aspiration cytology): Being done
for
pre-operative diagnosis of palpable orbital, eyelid
lesion
and
enlarged lymph node.
(III)
Clinical Pathology:
a)
Blood investigations are done with the help of Auto analyzer.
Hemogram
–
TLC-Total
Leukocyte count.
DLC-Differential
Leukocyte count.
ESR-
Erythrocyte Sedimentation Rate.
Platelet
count.
Peripheral
smear.
b)
Urine analysis –
Routine examination including urine for-
Protein
Sugar
Microscopy.
c)
Sample collection for all investigations at various Dr.
R. P.
Centre laboratories.
(IV) Immunohistochemistry:
Diagnostic and research purposes.
(V) Molecular
Pathology Laboratory
·
As a part of the Department of Ocular Pathology, this
section which was formally inaugurated in March 2009 undertakes
tests based on various molecular biology tools including
polymerase chain reaction, MSP, ISH for research purposes.
·
The research is presently related to cancer biology of
various ocular malignancies.
·
This section also provides training to undergraduates,
post graduate students in basic molecular biology techniques and
immunohistochemistry.
Museum:
Ocular
Pathology Museum was started by Prof. Ratnakar in 1976.
Interesting cases of lid, orbital lesion, intra ocular tumor
are still being preserved
for teaching purposes.
B.
Research work:
(a) Extramural
Research Projects:
1.
ICMR project on “Diagnosis of ocular and orbital round cell
tumours in children by Immunohistochemistry” (1989-91).
2.
ICMR project on “Immunophenotypic characterization of
ocular lymphoproliferative disorders” (1998-2001).
3.
ICMR project on “Prognostic markers in Retinoblastoma –
An immunohistochemical study” (Collaborative with Pediatric
Oncology and IRCH.) (2002-05).
4.
ICMR – “Detection of HPV genome in malignant ocular
lesions” (2008-ongoing)
(b) Research projects funded by All India Institute of Medical Sciences (AIIMS)
1.
“Human papilloma virus in conjunctival neoplasias”
(2000-01).
2.
“Immunohistochemical detection of HSV-1 in non- specific
keratitis” (2003-04).
3. “P53
expression in sebaceous gland carcinoma of the eye lid- An
immunohistochemical study” (2004-05).
4.
“Retinoblastoma protein (pRB) expression in human
eyes and ocular tumors(2005-06).
5.
“Immunophenotype characterization of small lymphocytic
lymphomas of the eye. (May-2007).”
6.
“p53 and mdm2 expression in orbital Rhabdomyosarcoma of
childhood and IHC.”
7.
Identification of Human Papilloma Virus types in
malignant ocular tumours (May
2009)
8.
Immunohistochemical detection of cancer stem cell markers in
retinoblastoma. ((may 2009)
(c)
Collaborative Research Projects:
1. An
open labeled, prospective study to evaluate the efficacy and
safety of ReliNethra (an autologous bio-engineered composite
limbal epithelial graft). (Reliance)-completed
2. Efficacy
and safety of Restasis in management of dry eye July 2006
(completed).
3. Culture
of Limbal stem cells for ocular surface reconstruction in stem cell
deficiency diseases (DBT)-2006.(completed)
4. Assesment
of Safety and efficacy of Relinethre TM – EAU (an
antilogous bioengineered conjunctiva) in the treatment of
conjunctival disorders (2008 onwards).
5. IH
and molecular studies on WntB-Catenin signaling pathway in sebaceous
cell carcinoma of the eye lid. (Ph.D.Thesis
– March
2007 onwards).
6. Isolation,
Expansion & characterization of corneal limbal epithelial
cells. (Ph.D.
Thesis – February 2006 onwards)
7. Identification
and characterization of differentially expressed gene in human
retinoblastoma-(PhD Thesis –March 2006 onwards).
8.
Expression of cell cycle regulatory proteins in Ocular
Surface Squamous Neoplasia (PhD Thesis February 2009)
Research
Publications:
1.
Kashyap S, Pushker N, Meel R, Sen S,
Bajaj MS et al Orbital
schwannoma with cystic degeneration. Clin and Exp
Ophthalmolo
2009;37(3): 293-8.
2.
Sen
S, Sharma A, Panda A. Indian J Ophthalmol.
2007,
55:361-3.
Immunohistochemical localization of human
papilloma virus in conjunctival neoplasias: A retrospective
study.
3.
Kashyap S, Sen S, Sharma MC, Betharia SM, Bajaj MS:
Alveolar soft part sarcoma of the orbit: report of three
cases.
Can J Ophthalmology 2004 ; 39 : 552-556.
4.
Sen S, Mahindrakar A, Betharia SM, Bajaj MS, Kashyap S,
Ghose S : Pleomorphic adenomas of the lacrimal gland:a
clinicipathological analysis : Clin Exp Ophthalmol 2004 ; 32:
523-525.
5.
Sen S, Venkatesh P, Moolchand.Primary Intraocular Hydatid
cyst with Glaucoma. J Pediatr Ophthalmol.Strabismus. 2003;
40 (5): 312-3.
6.
Sen S, Kashyap S, Singh UB, Nagasuresh V, Chand M, Garg
SP Intraocular tuberculosis mimicking
retinoblastoma-A case
report. Diagnostic Cytopathology 2003; 28: 107-109.
7.
Sen S, Kashyap S, S.M.Betharia SM: Primary Orbital
Plasmacytoma-A case report Orbit 2003; 22: 317-319.
8.
Sen S, Kashyap S, Thanikachalam S, Betharia SM: Primary
Primitive Neuroectodermal tumour of the orbit. Report of a
case. J Pediatr Ophthalmol Strabismus 2002; 39: 242-244.
9.
Kashyap S, Sen S, Sharma MC, Sethi A. Diagnostic intraocular
aspiration cytology of choroidal melanoma. Diagn. Cytopathol. 2002:
26: 389- 391.
10.
Kashyap S,Sen S,Betharia SM,Dade VK; Mesenchymal
chondrosarcoma of the orbit a clinicopathological study Orbit
2001:20;63-68.
11.
Kashyap S.kapila k,Verma K;Cytologic spectrum seen in fine
needle aspirates from Thyroglossal Cysts:J Cytol
2001:18;21-
24.
12.
Kashyap S: Intoacoular fine needle aspiration biopsy in
Retinoblastoma.DOS Times 2001:7;26.
13.
Kashyap S,Kapila K,Kumar N,Geeta G,Rath GK,Verma
K;Nucleolar
organizer regions and morphologic subtypes of
squamous
cell carcinoma of squamous cell carcinoma of
cervicx..Ind
J Pathol Microbiol 1998;41:303-308.
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