and antimicrobial sensitivity testing of bacteria
tissue culture facilities used for viral and Chlamydial culture
culure facility: This service is also provided for other
departments of AIIMS for CSF culture.
reference laboratory built under Indo-UK Colaboration. This
facility is also used by other departments of AIIMS and other
Institutions of the country.
Molecular biology laboratory
provided by the Ocular Microbiology Secttion:
Bacterial culture and antimicrobial sensitivity testing.
Culture for Acanthomoeba.
Preparation of in house culture medium for all the above.
Chlamydia antigen detection.
Chlamydia antigen production.
Herpes simplex virus antigen detection.
Viral isolation in tissue culture
PCR assays for bacteria, fungi, viruses and Chlamydia
1. Chlamydia laboratory at Dr RP Centre has culture facility along
with all other laboratory diagnostic facilities including
molecular diagnostics. It provides patient care service
facilities to various Departments of AIIMS:
R.P. Centre for Ophthalmic Sciences,
Deptt. of Obstetrics & Gynecology,
Dermatology & Venerology,
Deptt. of Pediatrics and
Deptt. of Medicine and many other Departments and other
Institutions in the country like: -
Shere- e- Kashmir Institute, Srinagar,
GTB Hospital & University College of Medical Sciences,
Sanjay Gandhi Post Graduate Institute, Lucknow & many other
Institutes. Till today more than 10,000 patient specimens have
2. This facility
was created over a period of 10 years with efforts of many in
India, viz. AIIMS, ICMR, Ministry of Health & FW and abroad like
WHO, Chlamydia reference Laboratory, London, Institute of
Ophthalmology, London and Overseas Development Authority, UK.
3. The laboratory is providing research and diagnostic
facilities for Chlamydial infections of male and female
reproductive tracts, respiratory infections and other systemic
infections. Work was done for determining the association of
Chlamydia pneumoniae with atherosclerosis with the help of
Cardiology Department of AIIMS. There are research projects with
the Deptt. Of Dermatology and Venerology, Obstretics & Gynaecology
in these aspects and the Lab technician is involved in these
EXTRAMURAL RESEARCH PROJECTS :
1. Indian Council of Medical Research (ICMR) ad hoc research
project: “Evaluation of polymerase chain reaction assay (PCR) for
detection of HSV genome in tear specimens as a rapid diagnostic
tool for laboratory diagnosis of HSV keratitis, particularly
stromal keratitis and atypical epithelial keratitis vis a vis
antigen detection and genomic DNA detection by PCR assay in
corneal scrapings:” .Started February 2007: For 3 years.
NMITLI ( New Milenium Technology Leadership Initiative) Project:
Novelmolecular diagnostics for eye diseases and low vision
enhancement devices.April 2003- Continuing
3. Indian Council of Medical Research (ICMR) ad hoc research
project. Standardisation of antifungal susceptibility testing
for filamentous fungi and characterisation of drug sensitive and
drug resistant fungal isolates in mycotic keratitis Apr2006-
4. ICMR ad-hoc
Research project: .Phenotypic and molecular characterisation of
ocular isolates of coagulase negative Staphylococci:implications
in colonuisation and virulenceJan. 2002 to April 2005
ad-hoc Research project : Molecular characterisation of
Streptococcus pneumoniae isolates causing various infections in
6. .Department of Biotechnology,
Govt. of India, project: Collection of well characterised
Chlamydiae isolates and development of suitable diagnostic assays.
April 2000- Maarch 2003
7. ICMR ad-hoc
Research project: Chlamydia pneumoniae and coronary artery
disease: Use of polymerase chain reaction assay (PCR), agent
detection and serology in investigating the association.March
2000- Feb. 2003
8. ICMR ad hoc
research project: Construction of genomic library of C
trachomatis serovar L2, identification and characterisation of
immunodominant recombinant clones.April 1996 – October 1999
9. Indo-UK project "Establishment of Chlamydia Reference
Centre at the RP Centre, AIIMS,
New Delhi. April
1993- March 1998
10. Indian Council of Medical Research (ICMR) ad hoc
research project: “ Development of sandwich ELISA method using
monoclonal antibodies for detection of Chlamydia antigen and it’s
evaluation vis-a vis indirect immunofluorescence assay in rapid
diagnosis of Chlamydial ocular and genital infections. April 1996-
Council of Medical Research (ICMR) ad hoc research project: “
Development and use of polymerase chain reaction assay (PCR) and
restriction fragment length pattern (RFLP) analysis of PCR
products for C trachomatis in epidemiology.”October 1993 to
Indian Council of Medical Research (ICMR) ad hoc research
project: “ Study of group specific antigens od C trachomatis
using monoclonal antibodies and use of such antibodies in
screening for acute follicular conjunctivitis caused by C
trachomatis.” August 1989 to August 1993.
project: Mycotic keratitis (1.4.84 to 31.3.1987).
Council of Medical Research (ICMR) ad hoc research project:
Medical management of keratomycosis (1.1.84 to 1.1.86).
RESEARCH PROJECTS FUNDED BY ALL INDIA INSTITUTE OF MEDICAL
1. All India Institute of Medical Sciences research project
“Characterisation of staphylococcus epidermidis in corneal ulcer –
a clinico – microbiological study” 1.4. 2000 to 31.3.2002
2. All India Institute of Medical Sciences research
project: “Acanthomoeba keratitis- a prospective study.”
April 1992- March 1993
3. All India
Institute of Medical Sciences research project: Comparision of
inclusion immunofluorescence assay with EIA using commercial kits
in serodiagnosis of trachoma.April 1990-March 1991
4. All India
Institute of Medical Sciences research project: “ Evaluation of
indirect immunofluorescence assay using monoclonal antibody in
rapid diagnosis of keratitis caused by Herpes Simplex Virus.
March 1989- April 1990
Collaborative Research Projects:
Population Council & Global Network for reproductive and perinatal
management of reproductive tract infections in India. An analysis
of clinical protocols and cost. Collaborative Department:
Department of Obstretics & Gynaecology, AIIMS. Completed2004
Project - Validation of alogarithms proposed by NACO for
syndromic management of urethral and vaginal discharge in a
tertiary care centre in northern India.Collaborative Department:
Department of Dermatology and Venerology, AIIMS.