For culture and antimicrobial sensitivity testing
Complete tissue culture facilities used for viral
and Chlamydial culture
Acanthomoeba culture facility: This service is
also provided for other departments of AIIMS for CSF culture.
Chlamydia reference laboratory built under
Indo-UK Colaboration. This facility is also used by other
departments of AIIMS and other Institutions of the country.
6. Molecular biology
Services provided by the Ocular Microbiology:
Bacterial culture and antimicrobial sensitivity
Culture and PCR for Acanthomoeba.
Preparation of in house culture medium for all the
Chlamydia antigen detection, serology, culture and
Herpes simplex virus antigen detection.
Viral isolation in tissue culture
PCR assays for bacteria, fungi, viruses , Chlamydia
Investigation of viral conjunctivitis epidemics (
determining the causative virus by culture, PCR and antigen
detection) which occur almost every year in Delhi
We process on an average 17,000 specimens per year
Research activities :
A total of 22
extramural research projects funded by DBT, ICMR,CSIR etc and Five
AIIMS funded projects were undertaken by the department till date.
Notable among them are the Indo-UK Collaborative project ( a
tripartite project between Overseas Development Authority,UK , ICMR
and Dr R P Centre) for the establishment of Chlamydia Reference
Laboratory at Dr R P Centre : and the CSIR NMITLI ( New Millenium
Technology Leadership Initiative ) Project for the development of
novel molecular diagnostics for eye diseases and low vision
enhancement devices , a DBT project for development of siRNA against
conjunctivitis causing viruses.
The regional reference laboratory for Chlamydial
infection was established under a grant in aid project with British
collaboration. Methods for culture, fluorescence detection,
molecular detection and serological detection methods were developed
and standardised . These were used to carry out extensive
epidemiological investigations with patients of follicular
conjunctivitis, cervicitis, sexually transmitted diseases and
artherosclerosis. Developed monoclonal antibodies against
Chlamydia trachomatis and developed an antigen detection ELISA
using it. the Major outer membrane protein of C. trachomatis
was cloned and expressed in E.Coli. Polymerase chain reaction
based assay for Chlamydia Pneumoni was developed .and
neonatal pneumonia was investigated. The lab. prepared a genomic
library of Chlamydia trachomatis and have fished out
immunoreactive clones from the library. These tests are now being
provided for several of the concerned Departments at the Institute.
Genotyping of C. trachomatis by ribotyping using
amplification of the 16S-23S rRNA gene followed by restriction
fragment length polymorphism and DNA sequencing was carried out.
These indicate difference between the strains infecting genital
organs from those infecting eye.
Besides these, molecular and serotyping of
Streptococcus pneumoniae isolates from eye infections along with
from systemic infections and commensals has been done and published.
Under a DBT project work siRNA was developed
against conjunctivitis causing viruses.
Under the CSIR NMITLI ( New Millenium Technology
Leadership Initiative ) programme in a multicentric collaborative
project we developed a DNA macrochip for simultaneous detection of
15 common pathogens causing eye & CNS infections. The chip has been
already patented in India and several other countries of Europe,
Asia and USA for eye & CNS infections.
Molecular and phenotypic methods have also been
developed and standardized for characterizing drug sensitive and
drug resistant mycelial fungal isolates in mycotic keratitis.
Slime producing bacteria like Staphylococcus
epidermidis in keratitis and intra-ocular device related
infections have been studied for their phenotypic and genotypic
characteristics in order to find out implications of these in the
pathogenesis of bacterial keratitis and device associated
Under an ICMR funded project, work is underway for
genotyping of Acanthomoeba isolates causing eye and CNS infections.
More than 8 PhD students have completed their PhD
from Ocular Microbiology and are working in responsible positions in
India and outside India. Currently 3 PhD students are pursuing their
PhD in Ocular Microbiology.
More than 270 National and International publications
have come out from the research works carried out in Ocular