Dr. Ashutosh Halder

Associate Professor

Department of Reproductive Biology

Dr. Ashutosh Halder                                                         MD, DNB, DM, MAMS                                                  Reproductive Genetics

Additional Professor                                                                                                                                                          Molecular Cytogenetics

                                                                                                                                                                                            Reproductive Endocrinology

                                                                                                                                                                                            Clinical Embryology

                                                                                            Teratology

                                                                                                                                                                                             Publications

Teaching Courses Guided

PhD (January & July sessions)

Training Provided

Long Term:                 Molecular Cytogenetics, Clinical Embryology, Reproductive Endocrinology, etc

National Workshop:    One Week Workshop on Molecular Cytogenetics as Annual Event

Services Provided

Andrology (Primary Testicular failure)                      

Molecular Cytogenetics

Research Conducted

Completed:

1.      Analysis for sperm aneuploidy of idiopathic oligoazoospermic & teratospermic men

2.      Evaluation of anembryonic pregnancy and early missed abortion product for chromosomal aneuploidy and uniparental disomy by molecular method

3.      Chromosome aneuploidy and mosaicism in preimplantation embryo (human)

4.       Chromosome aneuploidy and mosaicism in preimplantation embryo (mouse)

5.      Prevalence of 22q11 deletion syndrome in children with structural cardiac malformation: a tertiary care referral hospital-based study in Delhi

6.      Molecular Basis of Multiple Myeloma (collaborative)

Ongoing:

1.      Rapid Aneuploidy Diagnosis by PRINS

2.      Genetic & Endocrine Evaluation of Primary Testicular Failure

3.      Spermiation Defect

4.      Understanding Dynamics of Skewed Sex Ratio

5.      Molecular scanning for FISH negative 22q11.2 phenotypes

Visions

To develop reproductive genetics & reproductive onco-genetics

To develop semen cryopreservation (semen banking & AID) facility 

To develop gamete, embryo & gonad cryopreservation facility

To develop biobank and Reproductive Genetic Registry for future research

To develop clinical embryology

To start courses (teaching/training) on Molecular Cytogenetics, Reproductive Genetics, Clinical Embryology, Cryopreservation, etc

Publications

Published Full Article

1.      Haldar A, Sharma AK, Agarwal SS, et al. OEIS Complex with Cranio-Facial anomalies: Defect of Blastogenesis?  Am. J. Med. Genetics. 1994; 53: 21-23.

2.      Sharma AK, Haldar A, et al. Postmortem Radiography of perinatal deaths: an aid to Genetic Counseling. Indian Pediatrics. 1994; 31(6): 702-706.    

3.      Phadke SR, Haldhar A, et al.  GAPO Syndrome in a child without Dermal Hyaline Deposit. Am.  J. Med. Genetics. 1994; 51: 191-193.

4.      Sharma AK, Haldar A, et al. Preaxial Brachydactyly with Abduction of Thumbs & Hallux Varus: a distinct entity. Am. J. Med. Genetics. 1994; 49(3): 274-277.

5.      Sharma AK, Phadke SR, Haldar A, et al. Jercolevine Syndrome: a case report. Indian Pediatrics. 1994; 31(6): 707-708.    

6.      Sharma AK, Haldar A, et al. Marshall-Smith Syndrome: further observations. Indian Pediatrics. 1994; 31(8): 1098-1100.

7.      Buckshee K, Haldar A, et al. Bioeffects of Diagnostic Ultrasound in Newborn. J. Obst. & Gynecol India. 1994; 44(1): 859-862.

8.      Tutschek B, Sherlock J, Halder A, Delhanty J, Rodeck C, Adinolfi M. Isolation of Fetal Cells from transcervical samples by Micromanipulation: Molecular confirmation of their fetal origin and diagnosis of fetal aneuploidies. Prenatal Diagnosis. 1995; 15(10): 951-960.    

9.      Adinolfi M, Sherlock J, Tutschek B, Halder A, Delhanty J, Rodeck C. Detection of Fetal Cells in Transcervical Sample & Prenatal Diagnosis of Chromosomal abnormalities. Prenatal Diagnosis. 1995; 15(10): 943-950.

10.  Agarwal S, Kishore R, Haldar A, et al.  Outcome of pregnancy in women with Recurrent Spontaneous Abortion following Immunotherapy with Allogenic Lymphocytes. Human Reproduction.  1995; 10(9): 2280-2284.

11.  Kishore R, Agarwal S, Haldar A, et al.  HLA sharing, Antipaternal Cytotoxic Antibodies & MLR Blocking Factors in women with Recurrent Abortions. J. Obstet. Gynaecol. Res. 1996; 22(2): 177-183.    

12.  Sherlock J, Halder A, Tutschek B, Rodeck C, Adinolfi M. Prenatal detection of fetal aneuploidies using transcervical cell samples. J Med Genet. 1997; 34: 302-305.

13.  Halder A & Tutschek B.  Analysis of meiotic segregation in human nondecondensed interphase spermatozoa by multicolor rapid direct FISH. Ind J Med Res. 1998; 107: 94-97.

14.  Ruangvutilert P, Halder A, Jauniaux E, Arienzo M, Cirigliano V and Sherlock J.  A minimally invasive prenatal diagnosis technique for the collection of transcervical cells. Prenatal Neonatal Medicine 1998; 3: 294 - 296.

15.  Halder A, Pahi J, Pradhan M, Pandey A, Gujral R, Agarwal SS. Iniencephaly: a report of 19 cases.  Ind Pediatrics. 1998; 35: 891 - 896.

16.  Halder A, Pahi J, Sharma AK, Bhatia VL, Phadke RV, Gujral R & Agarwal SS. Osteodysplastic Primordial Dwarfism Type II with normal Mentation and delayed CNS Myelination. Am. J. Med. Genet. 1998; 80: 12-15.

17.  Pahi J, Phadke SR, Halder A, Agarwal SS, et al.  Does autopsy of antenataly diagnosed malformed fetuses aid genetic counseling? The National Medical Journal of India. 1998; 11(4): 120-121.

18.  Jauniaux E, Halder A, Partington C. A case of partial mole associated with trisomy 13. Ultrasound Obstet Gynaecol. 1998; 11(1): 62-64.

19.  Halder A & Park YK. Identification of the appropriate tissue from formalin fixed perinatal autopsy material for chromosomal ploidy detection by interphase FISH. Ind J Med Res. 1999; 110: 102-106.

20.  Phadke SR & Halder A. Fluorescent in situ hybridization: A novel method to study chromosomes and genes. Perinatology 2000; 2(4): 203-210.

21.  Halder A, Pahi J, Chadda V and Agarwal SS.  Sirenomelia Sequence associated with craniorachischisis totalis, limb reduction and primitive heart: a case report. Indian Pediatr. 2001; 38 (9): 1041-5.

22.  Halder A. Trisomy 21 beyond 1st trimester: are they all mosaic? Evidence to support. Asian J Obs & Gynae Practice, 2002; 6 (2): 30-34.

23.  Halder A, Agarwal S & Chaddha V. Genetic counseling in obstetric practice. Obstetrics & Gynecology Today 2002; VII (6): 310-318 (invited review).

24.  Chaddha V, Agarwal S, Phadke SR & Halder A (corresponding author). Low Level of Mosaicism in Atypical Prader Willi Syndrome: Detection using Fluorescent In Situ Hybridization. Indian Pediatrics 2003; 40: 166-168.

25.  Agarwal S, Chadda V, Ram Sharan and Halder A (corresponding author). Rapid prenatal diagnosis of trisomy 18 by interphase FISH. Asian Journal of Obs & Gynae Practice 2003; 7 (1): 14–15 & 23.

26.  Pandey MK, Halder A, Agarwal S, Srivastava M, Agarwal SS, Agrawal S. Immunotherapy in Recurrent Spontaneous Abortion: Randomised and Nonrandomised trials. The Internet Journal of Gynaecology and Obstetrics 2003; 2(1): rsa/xml (13 pages).

27.  Halder A, Panigrahi I & Pal L. Fowler like syndrome with extreme oligohydramnios & growth restriction and without muscle hypoplasia: first case from Indian subcontinent. Ind Pediat 2003; 40: 418-423.

28.  Halder A, Chaddha V, Agarwal S, Fauzdar A. Absence of sperm meiotic segregation error of chromosomes 1, 9, 12, 13, 16, 18, 21, X and Y in a case of 100 % necrozoospermia. Asian J Androl. 2003 Jun; 5(2): 163-166.

29.  Halder A, Halder S, Fauzdar A, Kumar A. Molecular approaches of chromosome analysis: an overview. Proc. Indian Nat. Sci. Acad. 2004; B70 (2): 153-221. Invited review

30.  Halder A, Agarwal S, Pandey A. Iniencephaly and Chromosome Mosaicism: A Report of Two Cases. Congenital Anomalies 2005; 45: 102-105.

31.  Halder A, Fauzdar A, Kumar A. Serum Inhibin B and Follicle Stimulating Hormone Levels as Markers in the Evaluation of Azoospermic Men: A Comparison. Andrologia 2005; 37: 173-179.

32.  Halder A, Fauzdar A, Kabra M and Saxena A. Detection of 22q11.2 hemizygous deletion by interphase FISH in a patient with features of CATCH22 Syndrome. Ind Pediatr. 2005; 42: 1236-1239.

33.  Halder A, Halder S, Fauzdar A. A Preliminary Investigation on Molecular Basis for Clinical Aggressiveness in Cervical Carcinoma by Comparative Genomic Hybridization and Conventional Fluorescent In-situ Hybridization. Indian J of Med Res. 2005; 122: 434-446.

34.  Halder A. Placental chimerism in early human pregnancy. Indian J of Human Genetics 2005; 11 (2): 84-88.

35.  Halder A and Fauzdar A. Extreme Skewing of Sex Ratio and Low Aneuploidy in Recurrent Early Missed Abortion. Ind J Med Res 2006; 124: 41-50.

36.  Halder A. Skewed Sex Ratio in India. Indian Journal of medical Research. 2006; 124: 583-584.

37.  Halder A, Fauzdar A. Potential use of blood, buccal and urine cells for rapid noninvasive diagnosis of suspected aneuploidy using FISH. Journal of Clinical & Diagnostic Research. 2007; 1 (2): 32-38.

38.  Halder A, Fauzdar A, Ghosh M, Kumar A. Serum Inhibin B: A Direct, Precise & Noninvasive Marker of Ovarian Function. Journal of Clinical & Diagnostic Research. 2007; 1(3): 131-137.

39.  Halder A. Nonimmunologic Hydrops Fetalis Associated with True Knot of Umbilical Cord: A Necropsy Study. J Turkish German Gynecol Assoc. 2007; 8(4): 420-423.

40.  Halder A. Unexplained Ten Consequetive Early Third Trimester Intrauterine Fetal Deaths: A Diagnostic Dilema. The Internet Journal of Gynecology and Obstetrics. 2007; Volume 7, Number 2. (IUD_0910.doc)

41.  Halder A. Premature Graying of Hairs, Premature Aging and Predisposition to Cancer in Jajjal, Punjab: A preliminary observation. Journal of Clinical & Diagnostic Research 2007; 6: 577-580.

42.  Halder A, Gupta RK. Male like external genitalia with epididymis in a case of 46,XX disorder of sex development due to  congenital adrenal hyperplasia. Journal of Research in Medical Sciences 2008; 13(3): 141-145.

43.  Halder A, Jain M, Kabra M, Gupta N. Mosaic 22q11.2 microdeletion syndrome: diagnosis and clinical manifestations of two cases.  Molecular Cytogenetics 2008; 1: 18.

44.  Fauzdar A, Sharma RK, Kumar A, Halder A. A Preliminary Study on Chromosome Aneuploidy and Mosaicism in Early Preimplantation Human Embryo by Fluorescence In Situ Hybridization. IJMR: 2008; 128 (3): 287-293

45.  Fauzdar A, Halder A, Kumar A. Effect of gonadotropins on chromosome aneuploidy, chromosome mosaicism and skewed sex ratio in mouse preimplantation embryos. IJMR 2009; 129: 669-675

46.  Yadav N, Sumod K, Kumar S, Jain M, Halder A, Saxena R, Mukhopadhyay A. Therapeutic effect of bone marrow-derived liver cells in phenotypic correction of murine hemophilia A. Blood; 2009 114: 4552-4561

47.  Shukla B, Agarwal S, Suri V, Pathak P, Sharma MC, Gupta D, Sharma BS, Suri A, Halder A, Sarkar C. Assessment of 1p/19q status by fluorescence in situ hybridization assay: A comparative study in oligodendroglial, mixed oligoastrocytic and astrocytic tumors. Neurol India 2009; 57: 559-566

48.  Halder A.  Amniotic Band Syndrome and/or Limb Body Wall Complex: Split or Lump. The Application of Clinical Genetics 2010; 3: 7-15

49.  Halder A.  46, XY Disorder of Sex Development with Mullerian Ducts Remnants. JCDR 2010; 4: 2169-2174

50.  Halder A, Jain M, Chaudhary I, Kabra M. Prevalence of 22q11.2 microdeletion in 121 patients with cardiac malformation.  BMC Medical Genetics 2010; 11: 101.

Published Abstract (Indexed Only)

1.      Detection & analysis of fetal cells in TCC using FISH & PCR amplification of unique fetal DNA sequences. Delhanty JDA, Sherlock J, Tutschek B, Halder A, Rodeck CH, Adinolfi M. Am. J. Hum. Genet. 1995; 57(4): A32 (159).

2.      Collection of fetal cells from cervical canal during 1st trimester of pregnancy: Sampling technique, molecular analysis and isolation of purely fetal material. Tutschek B, Sherlock J, Halder A, et al. Europe. J Hum. Genet. 1996; 4(suppl.1): 20 (1.014).

3.      Prenatal diagnosis of 4 Chromosome anomalies from TCC. Sherlock J, Tutschek B, Halder A, et al. Europ.J. Hum. Genet. 1996; 4 suppl.10: 126 (9.026).

4.      Analysis of meiotic segregation in human nondecondensed interphase spermatozoa by multi color directs FISH. Halder A, Tutschek B. J Med Genet. 1996; 33 (suppl.1); S48 (12.001).

5.      A minimally invasive prenatal diagnosis technique for the collection of transcervical cells. Ruangvutilert P, Halder A, Sherlock J, Jauniaux E, Anenzo MP, Adinolfi M. Am J Hum Genet 1997; 61(4) supplement: Abst. No. 924, Page A 161.

6.      Rapid detection of ploidy in paraffin embedded formalin fixed tissue in perinatal autopsy material by interphase FISH: a search for the right tissue. Halder A & Park YK. Am J Hum Genet 1998; 63(4) supplement: abstract No. 871.

7.      Sex Chromosome chimerism in early pregnancy: a report of 3 cases. Halder A, Park YK, et al. Eu J Hum Genet 1999; 7: P251 (May, Suppl.)

8.      Is all Down syndrome cases mosaic? Evidence to support. Halder A, Park YK, et al. Cytogent Cell Genet 1999; 85: P633 (July, Suppl.).

9.      Extreme Skewing of Sex Ratio in Recurrent Early Missed Abortions. Halder A, Fauzdar A & Kumar A. In Abstract Book of the 12th World Congress on Human Reproduction (Venezia, March 10-13, 2005); Electronic Publication (www.humanrep2005.org).

10.  Halder A, Halder S, Fauzdar A. A Preliminary Investigation on Molecular Basis for Clinical Aggressiveness in Cervical Carcinoma by Comparative Genomic Hybridization and Conventional Fluorescent In-situ Hybridization. J of Cancer Research & Therapeutics 2006; 2: S15.

Books/Book Chapters/Proceedings

1.      Advances in Human Cytogenetics: an overview. Edited by Halder A. As CME Monograph Series II of National Academy of Medical Sciences of India (funded by NAMS for publication & wider distribution) 2002

2.      Extreme Skewing of Sex Ratio in Recurrent Early Missed Abortions. Halder A, Fauzdar A & Kumar A. In Proceeding of the 12th World Congress on Human Reproduction (Venezia, March 10-13, 2005); Publisher CIC Edizioni Internazionali 2005; vol. 2 pp. 69-73.

3.      Does gonadotropins has any effect on chromosome aneuploidy, chromosome mosaicism and skewed sex ratio in mouse preimplantation embryos? Halder A, Fauzdar A, Kumar A. In Proceeding of the 1^st International Symposium on Gonadotropin (New Delhi, February 2008)

4.      Clinical & Molecular Cytogenetics. Halder A. In: API text book of medicine, 9^th edition (2010)

Miscelleneous

1.      Contribution in commercial syndrome data base computer software programme: London Dysmorphology Data Base, London, UK (LDDB)

2.      Contribution in commercial syndrome data base computer software program: Possum Database, Melbourne, Australia

Molecular Cytogenetics Facility

In-Charge: Dr. Ashutosh Halder, Associate Professor (Teaching Block, Second Floor, Room No. 2099, 2090C)

Email:             ashutoshhalder@gmail.com or ahalder@aiims.ac.in

Tel:                 011-26594211             Mobile:           09868397924/09313309579

Sample Collection, Storage & Transportation

For FISH

1 ml Heparinized & 1 ml EDTA blood in sterile condition

Transport to laboratory within 24 hours

Storage temperature & transportation temperature (if more than 6 hours) preferably between 5-10 *C

Semen/mouth wash/urine samples are preferred to be collected on site (laboratory)

Research

PRINS (Primed In Situ Labeling)

1. Rapid Aneuploidy Diagnosis (Chromosome X,Y,13, 18 & 21)

FISH

1. XY FISH for Gonosomal Mosaicism
2. Yq microdeletion by STS (multiplex PCR based)
3. Preimplantation Genetic Diagnosis (Chromosome 1, 9, 16, 18, X, Y for Human and Chromosome X, Y, 19, 1 for Mouse

Research & On Request

FISH with following clones from the BAC/PAC/YAC/Plasmid Library (vide table below)

BAC/PAC/YAC/ Plasmid Library (On Request and/or Research Work)

Alphoid/Repetitive Sequence

Locus Specific

Microdeletion & Microduplication Syndrome

Chromosome Paints

Mouse Clones