Dr. Parthaprasad Chattopadhyay

Additional Professor

Room No. 3037, Dept. of Biochemistry, PC Block 3^rd Floor,

All India Institute of Medical Sciences, New Delhi 110029, India

Tel: 011-26546748

E-mail: parthoaiims@hotmail.com; pchattopadhyay@aiims.ac.in

After graduating in Medicine from Calcutta University, I completed my post-graduation (MD) in Biochemistry followed by PhD from All India Institute of Medical Sciences. I joined the Faculty of this Institute in 1997 while working on the promoter status of c-myc and heterozygosity status of 17p13 chromosomal region (p53 and HIC1 loci) in human glial tumors.

Initially I also collaborated on work on intra-tumor genetic heterogeneity, p53 expression and DNA repair enzymes in glioblastomas. Later on I developed interest in the study of effect of reactive oxygen species on cancer chemotherapeutic cytoxicity in context of p53 status.

Since hypoxia is a frequent occurrence in solid tumors including gliomas and is a cause for derangement of ROS metabolism in cancer cells, my lab investigated the effect of hypoxia on the action of various drugs on cancer cell lines. Oct4 and Sox2 are genes that we have found to be induced by hypoxia and, hence, we are now working on the effect of hypoxia on cancer cell stemness and invasiveness with special reference to p53 and HIC1 expression.

My lab is also working on dsRNA mediated transcriptional gene silencing as well as Development of targeted therapeutics using alpha-fetoprotein and Placental like Alkaline Phosphatase in collaboration with Prof. Subrata Sinha of National Brain Research Centre, Manesar and Prof. D.P. Sarkar of Delhi University South Campus.

STUDENTS

i. MSc:

ii. MD: Dr. Muthuraman N

iii. PhD:

Mr. Mukesh Kumar –

Mr. Anand Singh – dsRNA mediated modulation of HIV-1 gene expression

Mr. Imran Khan – Use of Placental like Alkaline Phosphatase for tumor specific targeting

Mr. Mohd. Khalid Zakaria – Development of targeted therapeutics utilizing alpha-fetoprotein promoter

Mr. Muzzaffar Ahmad Kassab – Modulation of gene expression of E6 and E7 oncoproteins of HPV-18

Ms. Mohita Bhagat – Oxygen concentration, p53-HIC1 and stemness characteristics of glial tumor cell lines

RESEARCH & IMPORTANT PUBLICATIONS

a. Research interests –

1.      dsRNA mediated transcriptional gene silencing

2.      Development of targeted therapeutics using alpha-fetoprotein and Placental like Alkaline Phosphatase

3.      Cancer cell stemness and invasiveness modulation by hypoxia and p53-HIC1 axis

b. Publications –

1.      Palanichamy JK, Mehndiratta M, Bhagat M, Ramalingam P, Das B, Das P, Sinha S, Chattopadhyay P. (2010) .Silencing of integrated Human Papillomavirus-16 oncogenes by siRNA mediated heterochromatization..Mol Can Ther 2010;9(7): 2114-22.

2.      Pal A, Srivastava T, Sharma MK, Mehndiratta M, Das P, Sinha S, Chattopadhyay P. (2009). .Aberrant methylation and associated transcriptional mobilization of Alu elements contributes to genomic instability in hypoxia..J Cell Mol Med. 2009 Jun 5. [Epub ahead of print]

3.      Mondal AM, Chinnadurai S, Datta K, Chauhan SS, Sinha S, Chattopadhyay P. (2006) .Identification and functional characterization of a novel unspliced transcript variant of HIC-1 in human cancer cells exposed to adverse growth conditions..Cancer Res. 2006 Nov 1;66(21):10466-77.

4.      Datta K, Babbar P, Srivastava T, Sinha S, Chattopadhyay P. (2002) .p53 dependent apoptosis in glioma cell lines in response to hydrogen peroxide induced oxidative stress..Int J Biochem Cell Biol. 2002 Feb;34(2):148-57.

5.      Chattopadhyay P, Rathore A, Mathur M, Sarkar C, Mahapatra AK, Sinha S. (1997) .Loss of heterozygosity of a locus on 17p13.3, independent of p53, is associated with higher grades of astrocytic tumours..Oncogene. 1997 Aug 14;15(7):871-4.

6.      Datta K, Mathur SG, Srivastava T, Shah P, Chattopadhyay P, Sinha S. (2003) .Hydroxylamine potentiates the effect of low dose hydrogen peroxide in glioma cells independent of p53..Int J Biochem Cell Biol. 2003 Dec;35(12):1639-44.

7.      Chattopadhyay P, Banerjee M, Sarkar C, Mathur M, Mohapatra AK, Sinha S. (1995). Infrequent alteration of the c-myc gene in human glial tumours associated with increased numbers of c-myc positive cells..Oncogene. 1995 Dec 21;11(12):2711-4.

8.      Datta K, Shah P, Srivastava T, Mathur SG, Chattopadhyay P, Sinha S. (2004) .Sensitizing glioma cells to cisplatin by abrogating the p53 response with antisense oligonucleotides..Cancer Gene Ther. 2004 Aug;11(8):525-31.

9.      Mehndiratta  M, Palanichamy JK, Ramalingam P,  Pal A, Das P,  Sinha S, Chattopadhyay P. (2008) .Fluorescence acquisition during hybridization phase in quantitative real-time PCR improves specificity and signal-to-noise ratio..BioTechniques 2008 Dec;45(6): 625-634

10.  Chattopadhyay P and Sinha S. (1994).Protein homologies in an ever shrinking universe of ancestral exons.J Evol Biol. 1994 Nov. 7(6):757-9