RESEARCH

 

Dr. Y. D. Sharma

 

Awards

 

  1. S.S. Bhatnagar award (CSIR) for Mol. Biol. Of Malaria (1994).
  2. ICMR award (Dr. M.O.T. Iyangar Memorial Prize) for Malaria (1997).
  3.  Molecular Parasitology award of Academy of  Vector Borne Disease(2000).

Fellowships in Academies

 

  1. Fellow of Indian National Science Academy.
  2. Fellow of Indian Academy of Sciences, Bangalore.
  3. Fellow of National Academy of Sciences, India.
  4. Fellow of Indian Society for Malaria & other communicable Diseases.

 

Membership of International Science Academy and Societies

 

  1. Member of New York Academy of Sciences, USA.
  2. Member of American Society for Microbiology (ASM).
  3. Member of American Association for Advancement of Science (AAAS).
  4. Member of American Society  of Tropical Medicine Hyg.

  

Member of National Scientific Societies

 

  1. Society for Biological Chemists.
  2. Indian Immunology Society.
  3. Indian Society for Parasitology.
  4. National Academy of Sciences, India.
  5. National Academy of Environmental Science Academy.
  6. Academy of Biomedical Scientists.
  7. Molecular of Biomedical Scientists.
  8. Indian Society for Malaria and other commn. Diseases.
  9. National Academy of Vector Borne Diseases.

 

Advisory Committee Member

 

Member of large number of Advisory committees for several departments of Government of India as well as for several universities and research institutes.

 

Research Experience 

 

31 years Post Ph.D. research in Protein Chemistry and Molecular Biology. 

Since last 25 years working on Molecular parasitology.

 

Significant Contribution

 

1.            Identified multiple strains of P.falciparum in India.

2.            Isolated unique P.vivax antigens.

3.             Host-parasite interaction at molecular level.

4.            Molecular epidemiology of drug resistant malaria.

 

Publications

 

More than 100 in high impact journals.

 

 Conferences/Symposia attended

           

            Sessions Chaired in International Conferences  :    17

            Gordon Research Conferences, USA               :     4

            Guha Research Conference                             :     1

            Total International Conferences                         :    40

            Total National Conferences                               :    40

 

Research Supervision

 

Ph.D. Thesis

 

Completed  -  14

Ongoing      -   6

 

M. Biotech. Dissertations

 

As guide                                     

 

Completed  -  36                             

 

Others as guide -Two National Biotechnology Associates,

                  One INSA visiting Scientist,

                  One visiting scientist from UCMS on

                  National Medical Science Academy

                  20  Summer Trainees                  

      Post MD/MS trainees.

 

Post Graduate Teaching

 

M. Biotech

 

Teaching Molecular Biology and recombinant  DNA Technology since 1987.

 

Junior Residents and others:

 

Teaching Recombinant DNA Technology.

 

Publications (last five years)

 

1.                  R. Goswami, S. Goel, N. Tomar, N. Gupta, V. Lumb, Y.D. Sharma. Prevalence of clinical remission in patients with sporadic idiopathic hypoparathyroidism. Clin Endocrinol (Oxf) 10.1111/j.1365-2265.2009.03653.x. (2009)

 

2.         H. Bora, M.K. Das, A. Ahmed and Y.D. Sharma.  Variations in the mitochondrial DNA markers in the Anopheles (Cellia) sundaicus population from the Andaman and Nicobar Islands, India.  Acta Tropica. (2009)

 

3.         P.L. Mehndiratta, P. Bhalla, A. Ahmed and Y.D. Sharma. Molecular typing of methicillin-resistant Staphylococcus aureus strains by PCR-RFLP of SPA gene: a reference laboratory perspective. Indian J. Med. Microbiol., 27, 116-22 (2009).

 

4.         N. Valecha, P. Srivastava, S.S. Mohanty, P. Mittra, S.K. Sharma, P.K. Tyagi, K. Pradhan, Vas Dev, R. Singh, A.P. Dash, Y.D. Sharma. Therapeutic efficacy of artemether-lumefantrine in uncomplicated falciparum malaria in India. Malar. J., 8, 107 (2009).

 

5.         V. Lumb,  M. K. Das, P. Mittra, A. Ahmed, M. Kumar, P. Kaur, A. P. Dash, S. S. Singh and Y.D. Sharma. Emergence of an unusual sulfadoxine-pyrimethamine resistance pattern and a novel K540N mutation in dihydropteroate synthetase in Plasmodium falciparum isolates obtained from Car Nicobar Island, India, after the 2004 Tsunami. J. Infect. Dis., 199, 1064-73 (2009).

 

6.         V. Choudhary and Y.D. Sharma. Extensive heterozygosity in flanking microsatellites of Plasmodium falciparum Na(+)/H(+) exchanger (pfnhe-1) gene among Indian isolates. Acta Trop., 109, 241-4 (2009).

 

7.         A.Ahmed and Y.D. Sharma. Ribozyme cleavage of Plasmodium falciparum gyrase A gene transcript affects the parasite growth. Parasitol. Res., 103,751-63 (2008).

 

8.        M.T. Alam, H. Bora, M.K. Das and Y.D. Sharma. The type and mysorensis forms of the Anopheles stephensi (Diptera: Culicidae) in India exhibit identical ribosomal DNA ITS2 and domain-3 sequences. Parasitol. Res., 103, 75-80 (2008).

 

9.        M.T. Alam,  H. Bora, P. Mittra, N. Singh and Y.D. Sharma. Cellular immune responses to recombinant Plasmodium vivax tryptophan-rich antigen (PvTRAg) among individuals exposed to vivax malaria. Parasite Immunol., 30, 379-83 (2008).

 

10.       M.T. Alam, H. Bora, N. Singh and Y.D. Sharma. High immunogenecity and erythrocyte-binding activity in the tryptophan-rich domain (TRD) of the 74-kDa Plasmodium vivax alanine-tryptophan-rich antigen (PvATRAg74). Vaccine, 26, 3787-94 (2008).

 

11.       S. Garg, S.S. Chauhan, N. Singh and Y.D. Sharma. Immunological responses to a 39.8kDa Plasmodium vivax tryptophan-rich antigen (PvTRAg39.8) among humans. Microbes. Infect., 10, 1097-105 (2008).

 

12.       R.K. Mehlotra., G. Mattera, M.J. Bockarie, J.D. Maguire, J.K Baird, Y.D. Sharma, M. Alifrangis, G. Dorsey, P.J. Rosenthal, D.J. Fryauff, J.W. Kazura, M. Stoneking and Peter A.Zimmerman. Discordant patterns of genetic variation at two chloroquine-resistant loci in worldwide populations of malaria parasite Plasmodium falciparum. Antimicrob. Agents Chemother.,  52, 2212-22 (2008).  

 

13.       A. Siddiqui, H. Bora, N. Singh, A. P. Dash and Y. D. Sharma. Expression, purification, and characterization of the immunological response to a 40-kilodalton Plasmodium vivax tryptophan-rich antigen. Infect. Immun., 76, 2576-86 (2008).

 

14.       A. Thakur, M. T. Alam, H. Bora, P. Kaur and Y. D. Sharma. Plasmodium vivax: sequence polymorphism and effect of natural selection at apical membrane antigen 1 (PvAMA1) among Indian population. Gene, 419, 35-42 (2008). 

 

15.       A. Thakur, M. T. Alam and Y. D. Sharma. Genetic diversity in the C-terminal 42 kDa region of merozoite surface protein-1 of Plasmodium vivax (PvMSP-1(42)) among Indian isolates. Acta Trop., 108, 58-63 (2008).

 

16.        S. Vinayak, M. T. Alam, M. Upadhyay, M. K. Das, V. Dev, N. Singh, A. P. Dash and Y. D. Sharma. Extensive genetic diversity in the Plasmodium falciparum Na+/H+ exchanger 1 transporter protein implicated in quinine resistance. Antimicrob. Agents Chemother. 51, 4508-11 (2007).

 

17.       A. Siddiqui, N. Singh, and Y. D. Sharma. Expression and purification of a Plasmodium vivax antigen - PvTARAg55 tryptophan- and alanine-rich antigen and its immunological responses in human subjects. Vaccine, 26, 96-107 (2007).

 

18.       S. Garg, M. T. Alam, M. K. Das, V. Dev, A. Kumar, A. P. Dash, and Y. D. Sharma. Sequence diversity and natural selection at domain I of the apical membrane antigen 1 among Indian Plasmodium falciparum populations. Malar. J., 6, 154 (2007).

 

19.       D. S. Rawat, V. Lumb, Y. D. Sharma, S. T. Pasha and G. Singh. Histone as future drug target for malaria. J. Commun. Dis. 39, 119-28 (2007).

20.       S. Vinayak and Y.D. Sharma.  Inhibition of Plasmodium falciparum ispH (lytB) gene expression by hammerhead ribozyme.  Oligonucleotides 17, 189-200 (2007).

 

21.       M.T.  Alam, Richa Agarwal and Y.D. Sharma.  Extensive heterozygosity at four microsatellite loci flanking Plasmodium vivax dihydrofolate reductase gene.   Mol. Biochem. Parasitol., 153, 178-185 (2007).

 

22.       Asim A. Siddiqui, Rashmi Jalah and Y.D. Sharma.  Expression and purification of HtpX-like small heat shock integral membrane protease of an unknown organism related to Methylobacillus flagellatus. J. Biochem. Biophys. Methods., 70, 539-546 (2007).

 

23.       Jeana T DaRe, Rajeev K Mehlotra, Pascal Michon, Ivo Mueller, John Reeder, Y.D. Sharma, Mark Stoneking and Peter A Zimmerman.  Microsatellite polymorphism within pfcrt provides evidence of continuing evolution of chloroquine-resistant alleles in Papua New Guinea.  Malaria Journal, 6, 34 (2007).

 

24.        M.T. Alam, H. Bora, P.K. Bharti, M.A. Saifi, M.K. Das, Vas Dev, A. Kumar, N. Singh, A.P. Dash, B. Das, Wajihullah  and Y.D. Sharma.          Similar Trends of Pyrimethamine Resistance Associated Mutations in Plasmodium vivax and P. falciparum.  Antimicrobial. Agents Chemother,, 51, 857-863 (2007).

 

25.       M.T. Alam, M.K. Das, Vas Dev, M.A. Ansari and Y.D. Sharma.  Identification of two cryptic species in the Anopheles (Cellia) annularis complex using ribosomal DNA PCR-RFLP.  Parasitol Res., 100, 943-948 (2007).

 

26.       M.T. Alam, M.K. Das, Vas Dev, M.A. Ansari and Y.D. Sharma.  PCR-RFLP method for the identification of four members of the Anopheles annularis group of mosquitoes (Diptera: Culicidae).  Trans. R. Soc  Trop  Med. Hyg., 101, 239-244. (2007).

 

27.       Anwar Ahmed, Vanshika Lumb, Manoj K. Das, Vas Dev, Wajihullah  and Y.D. Sharma.  Prevalence of Mutations Associated with Higher Levels of  Sulfadoxine-Pyrimethamine Resistance in Plasmodium falciparum Isolates from Car Nicobar Island and Assam, India.  Antimicrobial. Agents Chemother. 50, 3934-3938. (2006).

 

28.       R. Sarin, N. Tomar, D. Ray, Y.D. Sharma and R. Goswami.  Absence of pathogenic calcium sensing receptor mutations in sporadic idiopathic hypopararthyroidism.  Clin. Endocinol. 65, 359-363. (2006).

 

29.      R. Sarin and Y.D. Sharma.  Thioredoxin system in obligate anaerobe Desulfovibrio desulfuricans: Identification and characterization of a novel thioredoxin 2.  Gene  376, 107-115. (2006).

 

30.       P. Mittra, S. Vinayak, H. Chandawat, M.K. Das, N. Singh, S. Biswas, Vas Dev, A. Kumar, M.A. Ansari and Y.D. Sharma.  Progressive increase in point mutations associated with chloroquine resistance in Plasmodium falciparum isolates from India.  J. Infect. Dis. 193, 1304-1312. (2006).

 

31.       A. Ahmed, M.K. Das, Vas Dev, M.A. Saifi, Wajihullah and Y.D. Sharma.  Quadruple mutations in dihydrofolate reductase of Plasmodium falciparum from Car Nicobar Island, India.  Antimicrobial. Agents Chemother. 50, 1546-1549. (2006).

 

32.       S. Vinayak, P. Mittra and Y.D. Sharma. Wide variation in microsatellite sequences within each Pfcrt mutant haplotype. Mol Biochem Parasitol. 147, 101-108. (2006).

 

33.       M.T. Alam, M.K. Das, M.A. Ansari and Y.D. Sharma.  Molecular identification of Anopheles (Cellia) sundaicus from the Andaman and Nicobar islands of India.  Acta. Trop.97, 10-18.  (2006).
           

34.       R. Jalah, R. Sarin, N. Sud, M.T. Alam, N. Parikh, T.K. Das and Y.D. Sharma.  Identification, expression, localization and serological characterization of a tryptophan-rich antigen from the human malaria parasite Plasmodium vivax.  Mol. Biochem. Parasitol. 142, 158-169.  (2005).

 

35.        Y.D. Sharma.  Genetic alteration in drug resistance markers of Plasmodium  falciparum. Ind. J. Med. Res. 121, 13-22 (2005).

 

36.       D.S. Rawat, I. Sharma, R. Jalah, S. Lomash, V. Kothekar, S.T. Pasha and Y.D. Sharma.  Identification, expression, modeled structure and serological characterization of Plasmodium vivax histone 2B.  Gene 337, 25-35 (2004).

 

37.       A. Ahmed, D. Bararia, S. Vinayak, M. Yamee, S. Biswas, V. Deb, A. Kumar, M.A. Ansari and Y.D. Sharma.  Plasmodium falciparum isolates in India exhibits a progressive increase in mutations associated with sulfadoxine-pyrimethamine resistance.  Antimicrobial. Agents Chemother.  48, 879-889 (2004).

 

 

Molecular Biology of Malaria Parasite (Group Leader - Prof. Y. D. Sharma)

 

For effective control of malaria, the vaccine and newer drugs are required. Prof. Sharma’s group isolated and characterized several immunobiologically important newer molecules from human malarial parasites Plasmodium falciparum and P.vivax. Among these molecules, they have identified a potential vaccine and drug targets.  They have also identified large number mutants of  P.falciparum strains in India and showed temporal increase in mutations in drug target genes of the parasite. These findings have their implications in malaria control programs such as vaccine and drug development.

 Dr. Jaya Sivaswami Tyagi

 

Education / Professional Studies:  I.S.C., Loreto Convent, Lucknow, 1969 (First class); B.Sc. from University of Delhi, 1973; M.Sc. from University of Delhi, 1975; Ph.D. from University of Delhi, 1979.

Membership of Professional Bodies: Society of Biological Chemists (India), Microbiological Society of India, Indian Society for Cell Biology, American Society of Microbiology.

 Institution

Post Held

Duration

V.P.Chest Institute, Delhi

 

JRF, SRF

 

Oct. 1975- July 1979

National Cancer Institute, NIH, Bethesda, MD, USA

 

International Visiting Fellow and International Visiting Associate, Dr. Ira Pastan’s laboratory

Aug. 1979 - June 1983

CSIR Centre for Biochemical Technology, Delhi (now IGIB)

Scientist C

June 1983- May 1987

AIIMS, New Delhi

Associate Professor

Additional Professor

Professor

June 1987- June 1991

July 1990-June 2001

July 2001- Present  

 

Awards, fellowships and membership of professional bodies:

·         National Science Talent Fellow, Indian Council of Agricultural Research. Scholarship during B.Sc, M.Sc. and fellowship during Ph.D.

·         First class First in B.Sc. (1973) and M.Sc. (1975), University of Delhi

·         First position in Biochemistry in the Agriculture Research Service (ARS) examination , 1978

·         Dr. Kona Sampath Kumar Memorial Prize, University of Delhi, 1985.

·         DBT Short-term Overseas Biotechnology Associateship, 1991. 

·         P.S. Sarma Memorial Award of the Society of Biological Chemists (India), 1999.

·         National Women Bioscientist Award, 1999.

·         New Millennium Science Medal, Indian Science Congress, 2000.

·         Tata Innovation Fellowship, Department of Biotechnology, Govt. of India, 2009

·         Member, Guha Research Conference, 1996.

·         Fellow, National Academy of Sciences, India, 1999.

·         Fellow, Indian Academy of Sciences, 2008


 

Key Activities:

Teaching:        Bacterial Genetics, Molecular Biology, Recombinant DNA Technology for Masters in Biotechnology

Training:         Summer students, PhD students supervisor and post MD/ MS trainees

Research:      Tuberculosis- molecular pathogenesis, dormancy, diagnosis, host pathogen interactions.

Other:            Project review for funding agencies, manuscript editorship activities for Journal of Biosciences and reviewing journal articles, involved in Institutional Patents Committee, Biosafety Committee and Research Committee work.

Current research efforts of Jaya S. Tyagi Lab:

1.       understanding the role of key dormancy regulating system, DevR-DevS/DosT (Rv3133c-Rv3132c/Rv2027c), in pathogenesis with special reference to dormancy and adaptation within macrophages

  1. developing and validating efficient diagnostic tools for pulmonary and extra pulmonary tuberculosis including TB meningitis. The USP multipurpose diagnostic technology has been developed which encompasses sample processing, highly sensitive smear microscopy, culture on solid/liquid medium and PCR. This technology is being licensed.

  2. exploiting the DevR-DevS two-component system as a novel antitubercular target.

  3. elucidating the mechanism of transcriptional activation mediated by DevR

  4. deciphering the environmental signals which DevS and Rv2027c sensor kinases respond to in vitro and macrophages

  5. evaluating host-M. tb  interactions in a novel dormancy model of infection.

 Most of the work has been published in peer-reviewed journals or has been filed for patents or are in press/ preparation.

Recent Publications (2005-2009):

  1. Chauhan S and Tyagi JS (2009) Powerful induction of tgs1-Rv3131 divergent genes in Mycobacterium tuberculosis is mediated by DevR interaction with a high affinity site and an adjacent cryptic low affinity site. J. Bacteriol. doi:10.1128/JB.00310-09.

  2. Chauhan S, Kumar A, Singhal A, Tyagi JS and Prasad HK (2009) CmtR, a cadmium-sensing ArsR–SmtB repressor, cooperatively interacts with multiple operator sites to autorepress its transcription in Mycobacterium tuberculosis. FEBS Journal 276:3428-39.

  3. Haldar S, Sharma N, Gupta VK and Tyagi JS (2009) Efficient diagnosis of tuberculous meningitis by detection of Mycobacterium tuberculosis DNA in cerebrospinal fluid filtrates using PCR. J Med Microbiol. 58: 616-624.

  4. Malhotra V, Tyagi JS & Clark-Curtiss J.E. (2009) DevR-mediated adaptive response in Mycobacterium tuberculosis H37Ra: Links to asparagine metabolismTuberculosis  Tuberculosis (Edinb). 89:169-74.

  5. Chauhan S & Tyagi JS (2008) Interaction of DevR with multiple binding sites synergistically activates divergent transcription of narK2-Rv1738 genes in Mycobacterium tuberculosis. J. Bacteriol. 190:5394-5403.

  6. Chauhan S & Tyagi JS (2008) Cooperative binding of phosphorylated DevR to upstream sites is necessary and sufficient for activation of the Rv3134c-devRS operon in Mycobacterium tuberculosis: Implication in the induction of DevR target genes. 190:4301-4312.

  7. Haldar S, Chakravorty S, Bhalla M, De Majumdar S. & Tyagi JS (2007) Simplified detection of Mycobacterium tuberculosis in sputum using smear microscopy and PCR with molecular beacons. J Med Microbiol. 56:1356-1362

  8. Pathak D, Chakravorty S, Hanif M & Tyagi JS (2007) Lysis of tubercle bacilli in fresh and stored sputum specimens: implications for diagnosing tuberculosis in stored and paucibacillary specimens by PCR. BMC Microbiology 2007, 7:83 doi:10.1186/1471-2180-7-83

  9. Taneja NK & Tyagi JS (2007) Resazurin reduction assays for screening of anti-tubercular compounds against dormant and actively growing Mycobacterium tuberculosis, Mycobacterium bovis BCG and Mycobacterium smegmatis. J Antimicrob Chemotherapy 60:288–293.

  10. Sharma D & Tyagi JS (2007) The value of comparative genomics in understanding mycobacterial virulence: Mycobacterium tuberculosis H37Ra genome sequencing – a worthwhile endeavour. J. Biosci. 32:185-189.

  11. Sharma D, Bose A, Shakila H, Das TK, Tyagi JS & Ramanathan VD (2006) Expression of mycobacterial cell division protein, FtsZ, and dormancy proteins, DevR and Acr, within lung granulomas throughout guinea pig infection. FEMS Immunol Med Microbiol 48:329-336.

  12. Tyagi JS (2006) The timeless legacy of Robert Koch. Resonance 11:20-28

  13. Chakravorty S, Pathak D, Dudeja M, Haldar S & Tyagi JS (2006) PCR amplification of shorter fragments from the devR (Rv3133c) gene significantly increases the sensitivity of tuberculosis diagnosis. FEMS Micro. Lett. 257: 306-311.

  14. Bagchi G, Chauhan S, Sharma D & Tyagi JS (2005) Transcription and autoregulation of the Rv3134c-devR-devS operon of Mycobacterium tuberculosis. Microbiology 151:4045-4053.

  15. Sen MK, Chakravorty S & Tyagi JS (2005) Polymerase chain reaction to identify Mycobacterium tuberculosis in patients with tuberculous lymphadenopathy. Natl. Medical J. India. 18:302-303.

  16. Chakravorty S, Sen MK & Tyagi JS (2005) Diagnosis of extrapulmonary tuberculosis by smear, culture and PCR using Universal Sample Processing technology. J. Clin. Microbiol. 43:4357-4362.

  17. Haldar S, De Majumdar S, Chakravorty S, Tyagi JS, Bhalla & Sen MK. (2005) Detection of acid-fast bacilli in postlysis debris of clinical specimens improves the reliability of PCR. J. Clin. Microbiol. 43: 3580-3581.

  18. Chakravorty S & Tyagi JS (2005) Novel Multipurpose Methodology for Detection of Mycobacteria in Pulmonary and Extrapulmonary Specimens by Smear Microscopy, Culture, and PCR. J. Clin. Microbiol. 43: 2697-2702.

  19. Chakravorty S, Dudeja M, Hanif M & Tyagi JS. (2005) Utility of Universal Sample Processing Methodology, Combining Smear Microscopy, Culture, and PCR, for Diagnosis of Pulmonary Tuberculosis. J. Clin. Microbiol. 43: 2703-2708

  20. Saini DK & Tyagi JS. (2005) High-throughput microplate phosphorylation assaysbased on DevR-DevS/Rv2027c 2-component signal transduction pathway to screen for novel antitubercular compounds. J. Biomol. Screening. 10:215-224.

 

 Patents:

     Indian: 5                               International: 6

Indian:

  1. A rapid, efficient, single - tube extraction procedure for the isolation of PCR – amplifiable M.tuberculosis DNA from clinical specimens, Appl. No. 497/ DEL/ 2000.

  2. A process for identifying and producing DevR protein of Mycobacterium tuberculosis has been granted, 1999.

  3. A process for identifying a novel target for the development of therapeutic modalities and drugs effective against tuberculosis, Appl. No. 1286/ DEL/ 2001.

  4. A simple and fast process for evaluating promoter activity of persistent M. tuberculosis in hypoxic conditions using M. smegmatis as a surrogate host. Appl. No. 981/DEL/2003.

  5. A method for diagnosis of tuberculosis by smear microscopy, culture and polymerase chain reaction using processed clinical samples and kit thereof.

International:

1.       A process for identifying a novel target for the development of therapeutic modalities and drugs effective against tuberculosis, Appl. No. PCT/IN02/00022,

2.       A process for identifying a novel target for the development of therapeutic modalities and drugs effective against tuberculosis. In national phase in US, UK and Japan.

3.       A screening method for developing drugs against pathogenic microbes having two-component system. US patent Appl. No. 60/418,837.

4.       A screening method for developing drugs against pathogenic microbes having two-component system. PCT Appl. No.PCT/IB03/04555.

5.       A method for diagnosis of tuberculosis by smear microscopy, culture and polymerase chain reaction using processed clinical samples and kit thereof. PCT Appl. No. PCT/IB 03/03211.

6.       A method for diagnosis of tuberculosis by smear microscopy, culture and polymerase chain reaction using processed clinical samples and kit thereof. In national phase in US, Europe, English speaking African countries, China.

 


 

Dr. H. K. Prasad

 

1.      Education:

 

M.Sc.   -   A.I.I.M.S., New Delhi; 1975

   Ph.D   -    A.I.I.M.S., New Delhi; 1981

 

2.      Fellowships:   Victor Hessier Post Doctoral Fellowship

                  DBT Short term over seas fellowship

 

3.      Elected Member:  Guha Research Conference 2006

 

4.      Member of Scientific Societies:

 

a.      Life member Indian Immunology Society

b.      Life member Molecular Immunology Forum

c.      Life Member Society of Biological Chemists, India

d.      Society for Scientific Values

e.      American Society for Microbiology

 

5.      Committee Member:

 

                a. Indian Council of Medical Research

b. National Research Development Council

c. National Institute of Biologicals

d. Department of Biotechnology, GOI

 

6.    Teaching / Research Experience:

 

Date

Location

Organization

Position

Responsibilities

1979-1981

New Delhi, India

All India Institute of Medical Sciences

Junior Research Fellow

Research

1981-1983

New York, USA

Heisser Foundation

Post Doctoral Fellow

Research

1983-1987

New Delhi, India

Indian Council of Medical Research

Senior Research Officer

Research & Teaching

1987-1988

New Delhi, India

All India Institute of Medical Sciences

Senior Research Officer

Research & Teaching

1988-1992

New Delhi, India

All India Institute of Medical Sciences

Associate Professor

Research & Teaching

1992-2001

New Delhi, India

All India Institute of Medical Sciences

Additional Professor

Research & Teaching

2001-

till date

New Delhi, India

All India Institute of Medical Sciences

Professor

Research & Teaching

 

 


 

7.      Significant Contribution:

 

--   Discovery and description of the HupB gene (Rv 2986c) of M. tuberculosis, a histone like DNA binding protein.

--   Differences between HupB gene (Rv 2986c) of M. tuberculosis and M. bovis (Mb 3010c) has been exploited for direct identification.

--   The development of a nested PCR assay for direct detection of M. tuberculosis and M. bovis in bovine and human clinical samples.

--   The potential possibilities of the occurrence of Zoonotic and reverse Zoonotic tuberculosis

--    Cytokine function generation and function in tuberculosis

 

8.      Publications:

 

Research Papers: 42                     

Others:  Chapter in Book: 3

International Patents:   Three          

 

9.      Conferences/Symposia attended:

 

      Sessions Chaired in International Conferences      :    5

      Total International Conferences                            :    10

      Total National Conferences                                  :    25

 

10. Research supervision:

 

Ph.D. Thesis

Completed  - 7

Ongoing      - 3

 

M. Biotech. Dissertations:   37

 

11. Key Activities:

 

               i.    Teaching      :     Immunology for Masters in Biotechnology

               ii.    Training       :     Summer students, guide for M. Biotechnology, PhD students and post MD/ MS trainees

               iii.    Research    :    Immunology of Tuberculosis; Diagnosis of Tuberculosis

 

12. Current research efforts: 

 

(i)  To establish the complex web of mycobacterial transmission that could potentially occur between humans and domesticated animals; and the reservoirs of infection that maintain the pathogens in the environment.  In order to establish transmission of pathogens from humans to cattle and cattle to humans efforts are on to identify and trace the origin of strains of mycobacterial pathogens isolated from clinical samples.

(ii)   Disregulation of interferon-gamma in patients of tuberculosis.

(iii) Characterization of the DNA binding protein of M. tuberculosis, Rv2986c.

 

 13. Future plans:

 

(i)        To understand the basis of tissue predilection of mycobacterial pathogens in clinical context

(ii)       Collaborative project for development of Bovine vaccines for prevention of tuberculosis.

 

14. Recent Publications:

 

1.    Visual format for detection of Mycobacterium tuberculosis and Mycobacterium bovis in clinical samples using molecular beacons. P. Kumar, K.Nath, B.Rath, M.K. Sen, P. Vishalakshi, D.S. Chauhan, V. M. Katoch, S. Singh, S. Tyagi, V. Sreenivas, H.K.Prasad. J. Molecular Diagnostics. J Mol Diagn 2009; 11:430–438.

2.    CmtR, a cadmium-sensing ArsR–SmtB repressor, cooperatively interacts with multiple operator sites to auto-repress its transcription in Mycobacterium tuberculosis.  Santosh Chauhan, Anil Kumar, A. Singhal, Jaya Sivaswami Tyagi and H. K. Prasad. FEBS Journal. 2009; 276:3428–3439.

3.    Association of tuberculous endometritis with infertility and other gynecological complaints of women in India. Kumar P, Shah NP, Singhal A, Chauhan DS, Katoch VM, Mittal S, Kumar S, Singh MK, Gupta SD, Prasad HK. J Clin Microbiol. 2008 Dec; 46(12):4068-70.

4.    Isolation of Mycobacterium bovis & M. tuberculosis from cattle of some farms in north India--possible relevance in human health. Srivastava K, Chauhan DS, Gupta P, Singh HB, Sharma VD, Yadav VS, Sreekumaran, Thakral SS, Dharamdheeran JS, Nigam P, Prasad HK, Katoch VM. Indian J Med Res. 2008 Jul; 128(1):26-31.

5.    Filtration of cerebrospinal fluid improves isolation of mycobacteria. Kumar P, Srivatsava MV, Singh S, Prasad HK. J Clin Microbiol. 2008 Aug; 46(8):2824-5.

 

6.    A  Singhal, A Jaiswal, VK Arora , HK Prasad.  Modulation of Interferon Gamma Receptor 1 By Mycobacterium tuberculosis: A potential immune evasive mechanism. Infection and Immunity, 2007; 75(5): 2500–2510.

 

 

Contact Information

 

E-mail:   hk_prasad@hotmail.com

Phone:   91-11-26594994; Fax: 91-11-26589286; 26588663

 Dr. S. N. Das

 Research highlight:

Various strategies are being tried by tumour biologist to modify tumour cells (transfection of non-pathogenic viral gene, cytokine genes, co-stimulatory molecules, oncogenes etc.) in order to develop tumour cell vaccine. These techniques have more disadvantages than advantages. My current area of research is related to physical and chemical modification of tumour cells from oral cancer patients and using it as stimulator cells for autologous Peripheral Blood Lymphocytes (PBL) and their functional and phenotypic characterization. Briefly, tumour cells are isolated from biopsy specimens of oral cancer patients, expanded in culture and modified by physical and chemical treatment. The modified tumour cells are used as stimulator cells to autologous PBLs obtained from the patients. Phenotypic and functional studies are done on the stimulated lymphocytes in terms of identification of predominant stimulated sub-sets of lymphocytes by flowcytometry using a panel of cell surface markers, like CD4, CD8, and CD16/56.  Functional studies of these cells are done in terms of cytokine production at RNA level by RT-PCR and at protein level by flowcytometry. Anti tumour activity of these activated PBLs on autologous tumour cells are being done by 51 Cr release assay and tumour neutralization assay.
 

          So far the results suggest that there is almost four fold enhanced proliferation of autologous PBLs when treated with modified tumour cells as compared to sam treated controls and these cells show Th-1 type of cytokine profile. These results prompted us to study in detail if these PBLs are capable of expressing transcription factors for cytokines and factors responsible for inducing apoptosis in the target cells by multi- colour flowcytometry and cDNA expression array.

 

Molecular Biology of Cancer (Mutation Analysis, Nucleotide sequencing):

 
BRCA-1 and BRCA-2 gene mutation have been shown to be associated with development of familial breast cancer syndrome (involving breast & ovarian cancer). Therefore, it could serve as a genetic marker for identification of high-risk groups. A detailed study of BRCA-1 and BRCA-2 gene mutation may also lead to development of molecular targets for these cancers. My group is also involved in the study of BRCA-1 and BRCA-2 gene mutation in breast cancer patients. We have screened a number of exons in BRCA-1and BRCA-2 locus for mutations in both familial and sporadic breast cancer patients. The mutations were further confirmed by nucleotide sequencing and reported a number of mutations and some novel mutations in this gene (Valarmathi et al. Human Mutation; 2003, 2004). We reported about 20 new mutations unique to Indian patients.

Current Research (Mutation analysis, DNA sequencing):

Current area of research includes molecular immunology and molecular biology of cancer and autoimmune endocrine diseases. We have been studying mutation/polymorphisms in some immune-regulatory (CTLA-4, Fas, Fas Land COX-2) genes in patients with oral cancer, breast cancer and diabetes. Further work in BRCA1 and BRCA2 genes are continuing. 

Research grants (current):

 

 

Source of funding

 

Title of the project

 

Duration

 

Amount

(Rs.)

ICMR

Studies on Fas (CD 95) and FasL (CD95L) gene polymorphisms in oral cancer patients

3 years

(2008 – 10)

6.0 lakh

DBT

Development & characterization of dendritic cell based NKT cell vaccine for oral cancer

3 years

(2007 – 09)

23.0 lakh

 

(Research work being undertaken by the faculty of the department)

 

Dr. Anushree Gupta 

 

Dr Gupta joined the Department of Biotechnology as Assistant Professor in July 2003. She is involved with the setting up of the bioinformatics section of the department with following objectives:

  1. To develop opportunities for students to understand the theory and application of computational methods of analysis.

  2. To promote collaborations between experimental biologists and computational biologists.

  3. To discuss new approaches to computational problems that arise in basic biological research.

  4. To submit proposals for funding bioinformatics teaching and research.